Separation of ketoprofen enantiomers at nanomolar concentration levels by micellar electrokinetic chromatography with on-line electrokinetic preconcentration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33147305" target="_blank" >RIV/61989592:15310/13:33147305 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2478/s11532-012-0177-0" target="_blank" >http://dx.doi.org/10.2478/s11532-012-0177-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11532-012-0177-0" target="_blank" >10.2478/s11532-012-0177-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Separation of ketoprofen enantiomers at nanomolar concentration levels by micellar electrokinetic chromatography with on-line electrokinetic preconcentration

Popis výsledku v původním jazyce

Separation of enantiomers represents an extremely important task in the field of analytical chemistry. This paper contributes to the field of the on-line preconcentration of enantiomers by developing a novel setup based on the electrokinetic accumulationof ketoprofen enantiomers on the pH boundary followed by enantioselective mobilization by a mixture of SDS, sulfated-beta-cyclodextrin (S-beta-CD), and trimethyl-beta-cyclodextrin (TM-beta-CD). Under the best conditions, where the injection electrolytewas composed of 50 mmol L-1 borate/NaOH pH 9.5 with 60% (v/v) methanol, the background electrolyte contained 50 mmol L-1 phosphate/NaOH pH 2.5, and the mobilization electrolyte consisted of 50 mmol L-1 phosphate/NaOH pH 2.5 with 4.0% (w/v) S-beta-CD, 0.5% (w/v) TM-beta-CD, and 20 mmol L-1 SDS, the determination of nanomolar concentration levels of ketoprofen enantiomers was successful by using micellar electrokinetic chromatography with a common UV detection. LODs were 2.5 nmol L-1 and 3

Název v anglickém jazyce

Separation of ketoprofen enantiomers at nanomolar concentration levels by micellar electrokinetic chromatography with on-line electrokinetic preconcentration

Popis výsledku anglicky

Separation of enantiomers represents an extremely important task in the field of analytical chemistry. This paper contributes to the field of the on-line preconcentration of enantiomers by developing a novel setup based on the electrokinetic accumulationof ketoprofen enantiomers on the pH boundary followed by enantioselective mobilization by a mixture of SDS, sulfated-beta-cyclodextrin (S-beta-CD), and trimethyl-beta-cyclodextrin (TM-beta-CD). Under the best conditions, where the injection electrolytewas composed of 50 mmol L-1 borate/NaOH pH 9.5 with 60% (v/v) methanol, the background electrolyte contained 50 mmol L-1 phosphate/NaOH pH 2.5, and the mobilization electrolyte consisted of 50 mmol L-1 phosphate/NaOH pH 2.5 with 4.0% (w/v) S-beta-CD, 0.5% (w/v) TM-beta-CD, and 20 mmol L-1 SDS, the determination of nanomolar concentration levels of ketoprofen enantiomers was successful by using micellar electrokinetic chromatography with a common UV detection. LODs were 2.5 nmol L-1 and 3

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Central European Journal of Chemistry

ISSN

1895-1066

e-ISSN

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Svazek periodika

11

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

6

Strana od-do

335-340

Kód UT WoS článku

000312878400003

EID výsledku v databázi Scopus

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