Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33147733" target="_blank" >RIV/61989592:15310/13:33147733 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/13:00398937

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jsfa.5930/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jsfa.5930/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jsfa.5930" target="_blank" >10.1002/jsfa.5930</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation

Popis výsledku v původním jazyce

Background Food and feed technology and biotechnology benefit from the use of immobilised enzymes. New low-cost enzyme carriers exhibiting high biocompatibility and response to external magnetic field can substantially improve the application potential of immobilised enzyme systems. Results Ferrofluid-modified spent grain was used as a low-cost, biocompatible and magnetically responsive carrier for the immobilisation of Candida rugosa lipase. Several immobilisation procedures were tested using both native and poly(ethyleneimine)-modified magnetic spent grain. Activity of immobilised lipase per unit mass of carrier, operational stability, time stability and Michaelis constant were compared. In general, magnetic spent grain modified with poly(ethyleneimine) bound a smaller amount of active lipase than unmodified magnetic spent grain, but the operational and storage stabilities of enzyme immobilised on poly(ethyleneimine)-modified carrier were very high. Conclusion Ferrofluid-modified spe

Název v anglickém jazyce

Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation

Popis výsledku anglicky

Background Food and feed technology and biotechnology benefit from the use of immobilised enzymes. New low-cost enzyme carriers exhibiting high biocompatibility and response to external magnetic field can substantially improve the application potential of immobilised enzyme systems. Results Ferrofluid-modified spent grain was used as a low-cost, biocompatible and magnetically responsive carrier for the immobilisation of Candida rugosa lipase. Several immobilisation procedures were tested using both native and poly(ethyleneimine)-modified magnetic spent grain. Activity of immobilised lipase per unit mass of carrier, operational stability, time stability and Michaelis constant were compared. In general, magnetic spent grain modified with poly(ethyleneimine) bound a smaller amount of active lipase than unmodified magnetic spent grain, but the operational and storage stabilities of enzyme immobilised on poly(ethyleneimine)-modified carrier were very high. Conclusion Ferrofluid-modified spe

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of the Science of Food and Agriculture

ISSN

0022-5142

e-ISSN

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Svazek periodika

93

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

1598-1602

Kód UT WoS článku

000317614000010

EID výsledku v databázi Scopus

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