In vitro characterisation of the anti-intravasative properties of the marine product heteronemin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33148029" target="_blank" >RIV/61989592:15310/13:33148029 - isvavai.cz</a>

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs00204-013-1045-1" target="_blank" >http://link.springer.com/article/10.1007%2Fs00204-013-1045-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00204-013-1045-1" target="_blank" >10.1007/s00204-013-1045-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro characterisation of the anti-intravasative properties of the marine product heteronemin

Popis výsledku v původním jazyce

Metastases destroy the function of infested organs and are the main reason of cancer-related mortality. Heteronemin, a natural product derived from a marine sponge, was tested in vitro regarding its properties to prevent tumour cell intravasation throughthe lymph-endothelial barrier. In three-dimensional (3D) cell cultures consisting of MCF-7 breast cancer cell spheroids that were placed on lymph-endothelial cell (LEC) monolayers, tumour cell spheroids induce "circular chemorepellent-induced defects" (CCIDs) in the LEC monolayer; 12(S)-Hydroxyeicosatetraenoic acid (12(S)-HETE) and NF-kappa B activity are major factors inducing CCIDs, which are entry gates for tumour emboli intravasating the vasculature. This 3D co-culture is a validated model for theinvestigation of intravasation mechanisms and of drugs preventing CCID formation and hence lymph node metastasis. Furthermore, Western blot analyses, NF-kappa B reporter, EROD, SELE, 12(S)-HETE, and adhesion assays were performed to inves

Název v anglickém jazyce

In vitro characterisation of the anti-intravasative properties of the marine product heteronemin

Popis výsledku anglicky

Metastases destroy the function of infested organs and are the main reason of cancer-related mortality. Heteronemin, a natural product derived from a marine sponge, was tested in vitro regarding its properties to prevent tumour cell intravasation throughthe lymph-endothelial barrier. In three-dimensional (3D) cell cultures consisting of MCF-7 breast cancer cell spheroids that were placed on lymph-endothelial cell (LEC) monolayers, tumour cell spheroids induce "circular chemorepellent-induced defects" (CCIDs) in the LEC monolayer; 12(S)-Hydroxyeicosatetraenoic acid (12(S)-HETE) and NF-kappa B activity are major factors inducing CCIDs, which are entry gates for tumour emboli intravasating the vasculature. This 3D co-culture is a validated model for theinvestigation of intravasation mechanisms and of drugs preventing CCID formation and hence lymph node metastasis. Furthermore, Western blot analyses, NF-kappa B reporter, EROD, SELE, 12(S)-HETE, and adhesion assays were performed to inves

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

<a href="/cs/project/ED0007%2F01%2F01" target="_blank" >ED0007/01/01: Centrum regionu Haná pro biotechnologický a zemědělský výzkum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Toxicology

ISSN

0340-5761

e-ISSN

—

Svazek periodika

87

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

1851-1861

Kód UT WoS článku

000324773300008

EID výsledku v databázi Scopus

—