Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151286" target="_blank" >RIV/61989592:15310/14:33151286 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-1420-3_3" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-1420-3_3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-1420-3_3" target="_blank" >10.1007/978-1-4939-1420-3_3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs

Popis výsledku v původním jazyce

Tip growth is one of the most preferable models in the study of plant cell polarity; cell wall deposition is restricted mainly to a certain area of the cell, and cell expansion at this specific area leads to the development of tubular outgrowth. Tip-growing root hairs are well-established systems for such studies, because their lateral position within the root makes them easily accessible for experimental approaches and microscopic observations. Fundamental structural and molecular processes driving tipgrowth are exocytosis, endocytosis, and all aspects of vesicular and endosomal dynamic trafficking, as related to targeted membrane flow. Study of vesicles and endosomes in living root hairs, however, is rather difficult, due to their small size and dueto the resolution limits of conventional light microscopes. Here we present noninvasive approaches for visualizing vesicular and endosomal compartments in the tip of growing root hairs using electronic light microscopy, contrast-enhanced

Název v anglickém jazyce

Live Microscopy Analysis of Endosomes and Vesicles in Tip-Growing Root Hairs

Popis výsledku anglicky

Tip growth is one of the most preferable models in the study of plant cell polarity; cell wall deposition is restricted mainly to a certain area of the cell, and cell expansion at this specific area leads to the development of tubular outgrowth. Tip-growing root hairs are well-established systems for such studies, because their lateral position within the root makes them easily accessible for experimental approaches and microscopic observations. Fundamental structural and molecular processes driving tipgrowth are exocytosis, endocytosis, and all aspects of vesicular and endosomal dynamic trafficking, as related to targeted membrane flow. Study of vesicles and endosomes in living root hairs, however, is rather difficult, due to their small size and dueto the resolution limits of conventional light microscopes. Here we present noninvasive approaches for visualizing vesicular and endosomal compartments in the tip of growing root hairs using electronic light microscopy, contrast-enhanced

Druh

C - Kapitola v odborné knize

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

<a href="/cs/project/LO1204" target="_blank" >LO1204: Udržitelný rozvoj výzkumu v Centru regionu Haná</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Endosomes: Methods and Protocols

ISBN

978-1-4939-1419-7

Počet stran výsledku

14

Strana od-do

31-44

Počet stran knihy

286

Název nakladatele

Humana Press

Místo vydání

Clifton

Kód UT WoS kapitoly

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