Retention behavior of a homologous series and positional isomers of aliphatic amino acids in hydrophilic interaction chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33151680" target="_blank" >RIV/61989592:15310/14:33151680 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/14:10282195

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201301348/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201301348/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201301348" target="_blank" >10.1002/jssc.201301348</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Retention behavior of a homologous series and positional isomers of aliphatic amino acids in hydrophilic interaction chromatography

Popis výsledku v původním jazyce

The retention behavior of several series of free - and -amino acids and positional isomers of amino pentanoic acid in the hydrophilic interaction chromatography mode (HILIC) was studied. The study was carried out on three stationary phases followed by post-column derivatization with fluorescence detection in order to describe the retention mechanism of the tested amino acids. The effect of chromatographic conditions including acetonitrile content in the mobile phase, mobile phase pH (ranging from 3.5 to6.5) and concentration of buffer in the mobile phase was investigated. The effect of the number of carbon atoms (n(C)) in aliphatic chains of the individual homologue of - and -amino acids and the logarithm of the partition coefficient (logD) on retention was also a part of the presented study. A good correlation (r } 0.98) between the logk and logD values of amino acids or n(C), respectively, was observed. The described linear relationships were subsequently applied to predict the rete

Název v anglickém jazyce

Retention behavior of a homologous series and positional isomers of aliphatic amino acids in hydrophilic interaction chromatography

Popis výsledku anglicky

The retention behavior of several series of free - and -amino acids and positional isomers of amino pentanoic acid in the hydrophilic interaction chromatography mode (HILIC) was studied. The study was carried out on three stationary phases followed by post-column derivatization with fluorescence detection in order to describe the retention mechanism of the tested amino acids. The effect of chromatographic conditions including acetonitrile content in the mobile phase, mobile phase pH (ranging from 3.5 to6.5) and concentration of buffer in the mobile phase was investigated. The effect of the number of carbon atoms (n(C)) in aliphatic chains of the individual homologue of - and -amino acids and the logarithm of the partition coefficient (logD) on retention was also a part of the presented study. A good correlation (r } 0.98) between the logk and logD values of amino acids or n(C), respectively, was observed. The described linear relationships were subsequently applied to predict the rete

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Svazek periodika

37

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

739-747

Kód UT WoS článku

000333538600001

EID výsledku v databázi Scopus

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