Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33153248" target="_blank" >RIV/61989592:15310/14:33153248 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-4939-0922-3_2" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-0922-3_2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-0922-3_2" target="_blank" >10.1007/978-1-4939-0922-3_2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR

Popis výsledku v původním jazyce

Although it is generally accepted that signal transduction in plant mitogen-activated protein kinase signaling cascades is regulated via rapid posttranslational modifications, there are also several compelling examples of swift stress induced transcriptional activation of plant MAP kinase genes. A possible function of these fast and transient events is to compensate for protein losses caused by degradation of phosphorylated MAP kinases within stimulated pathways. Nevertheless, there is still need for additional evidence to precisely describe the regulatory role of plant MAP kinase transcriptional dynamics, especially in the context of whole stress stimulated pathways including also other signaling molecules and transcription factors. During the last two decades a reverse transcription quantitative real-time PCR became a golden choice for the accurate and fast quantification of the gene expression and gene expression dynamic. In here, we provide a robust, cost-effective SYBR Green-based

Název v anglickém jazyce

Quantification of Stress-Induced Mitogen-Activated Protein Kinase Expressional Dynamic Using Reverse Transcription Quantitative Real-Time PCR

Popis výsledku anglicky

Although it is generally accepted that signal transduction in plant mitogen-activated protein kinase signaling cascades is regulated via rapid posttranslational modifications, there are also several compelling examples of swift stress induced transcriptional activation of plant MAP kinase genes. A possible function of these fast and transient events is to compensate for protein losses caused by degradation of phosphorylated MAP kinases within stimulated pathways. Nevertheless, there is still need for additional evidence to precisely describe the regulatory role of plant MAP kinase transcriptional dynamics, especially in the context of whole stress stimulated pathways including also other signaling molecules and transcription factors. During the last two decades a reverse transcription quantitative real-time PCR became a golden choice for the accurate and fast quantification of the gene expression and gene expression dynamic. In here, we provide a robust, cost-effective SYBR Green-based

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant MAP Kinases: Methods and Protocols

ISBN

978-1-4939-0921-6

Počet stran výsledku

13

Strana od-do

13-25

Počet stran knihy

266

Název nakladatele

Humana Press

Místo vydání

Clifton

Kód UT WoS kapitoly

—