Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33153560" target="_blank" >RIV/61989592:15310/14:33153560 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/14:00440405 RIV/61389030:_____/14:00440405 RIV/62156489:43210/14:00216922

Výsledek na webu

<a href="http://download.springer.com/static/pdf/897/art%253A10.1007%252Fs00709-014-0644-x.pdf?auth66=1425563607_400ef2156c697438d32da5554b1833db&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/897/art%253A10.1007%252Fs00709-014-0644-x.pdf?auth66=1425563607_400ef2156c697438d32da5554b1833db&ext=.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00709-014-0644-x" target="_blank" >10.1007/s00709-014-0644-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes

Popis výsledku v původním jazyce

Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse theMT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selectedecotypes under different copper treatments to show the tissue-specific expression response to copper stress. We demonstrated that higher copper concentrations specifically affected MT3 expression among ecotypes. Our analysis shows that MT

Název v anglickém jazyce

Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes

Popis výsledku anglicky

Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse theMT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selectedecotypes under different copper treatments to show the tissue-specific expression response to copper stress. We demonstrated that higher copper concentrations specifically affected MT3 expression among ecotypes. Our analysis shows that MT

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Protoplasma: an international journal of cell biology

ISSN

0033-183X

e-ISSN

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Svazek periodika

251

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

13

Strana od-do

1427-1439

Kód UT WoS článku

000344336500013

EID výsledku v databázi Scopus

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