Fast Cell Counting - The Better Cell Counting?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33156327" target="_blank" >RIV/61989592:15310/14:33156327 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fast Cell Counting - The Better Cell Counting?

Popis výsledku v původním jazyce

A better understanding of environmental factors influencing human health will benefit from the availability of fast and reliable methods allowing the quasi simultaneous measurement of a panel of endpoints in cell exposure studies. Automated cell-counting methods are basic for this purpose if comparability with traditional manual cell counting procedure is documented. In the present paper, both dead and viable cells of human lung adenocarcinoma epithelial cell line (A549) and human bronchial epithelial cell line (16HBE 14o-) at various cell densities ranging from 50 000 to 400 000 cells/ml, are counted by both Trypan blue dye exclusion staining counted with a hemo-cytometer and a handheld automated cell counter based on the Coulter principle (Scepter 2.0, Millipore). Correlation indexes higher than 0.998 have been obtained using both cell counting protocols in all cases, thus confirming the reliable use of automated cell counters whenever fast, easy to use, precise cell counting is a requisite.

Název v anglickém jazyce

Fast Cell Counting - The Better Cell Counting?

Popis výsledku anglicky

A better understanding of environmental factors influencing human health will benefit from the availability of fast and reliable methods allowing the quasi simultaneous measurement of a panel of endpoints in cell exposure studies. Automated cell-counting methods are basic for this purpose if comparability with traditional manual cell counting procedure is documented. In the present paper, both dead and viable cells of human lung adenocarcinoma epithelial cell line (A549) and human bronchial epithelial cell line (16HBE 14o-) at various cell densities ranging from 50 000 to 400 000 cells/ml, are counted by both Trypan blue dye exclusion staining counted with a hemo-cytometer and a handheld automated cell counter based on the Coulter principle (Scepter 2.0, Millipore). Correlation indexes higher than 0.998 have been obtained using both cell counting protocols in all cases, thus confirming the reliable use of automated cell counters whenever fast, easy to use, precise cell counting is a requisite.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/EE2.3.30.0004" target="_blank" >EE2.3.30.0004: Podpora vytváření excelentních výzkumných týmů a intersektorální mobility na Univerzitě Palackého v Olomouci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fresenius Environmental Bulletin

ISSN

1018-4619

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

3054-3058

Kód UT WoS článku

000348744700015

EID výsledku v databázi Scopus

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