Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33156921" target="_blank" >RIV/61989592:15310/15:33156921 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.15255/CABEQ.2014.2079" target="_blank" >http://dx.doi.org/10.15255/CABEQ.2014.2079</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15255/CABEQ.2014.2079" target="_blank" >10.15255/CABEQ.2014.2079</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes
Popis výsledku v původním jazyce
Laccase from Trametes versicolor was immobilized on magnetic (Fe3O4) nanoparticles and microparticles. Free and immobilized laccase had activities 163 and about 4.6 nkat mg(-1) respectively. Immobilized was 125.8 mu g enzyme mg(-1) of nanoparticles and 13 mu g enzyme mg(-1) of microparticles. The K-m were 10.55, 9.02 and 11.14 mmol L-1 for free laccase, immobilized on nanoparticles and immobilized on microparticles. The pH optimum after immobilization was about 0.5 pH less than before immobilization. Immobilized laccase retained 55 % (nano) and 47 % (micro) of initial activity after nine repetitions. Values of T-50 calculated respectively for free laccase, immobilized on nanoparticles and microparticles at 54.5 degrees C, 57.5 degrees C and 46 degreesC. Dyes Direct Blue 78, Acid Blue 225, Reactive Red 195, Acid Blue 74, and Phenol Red were used for decolorization by free and immobilized laccase. Laccase was able to decolorize most of the dyes well.
Název v anglickém jazyce
Immobilization of Laccase on Magnetic Carriers and Its Use in Decolorization of Dyes
Popis výsledku anglicky
Laccase from Trametes versicolor was immobilized on magnetic (Fe3O4) nanoparticles and microparticles. Free and immobilized laccase had activities 163 and about 4.6 nkat mg(-1) respectively. Immobilized was 125.8 mu g enzyme mg(-1) of nanoparticles and 13 mu g enzyme mg(-1) of microparticles. The K-m were 10.55, 9.02 and 11.14 mmol L-1 for free laccase, immobilized on nanoparticles and immobilized on microparticles. The pH optimum after immobilization was about 0.5 pH less than before immobilization. Immobilized laccase retained 55 % (nano) and 47 % (micro) of initial activity after nine repetitions. Values of T-50 calculated respectively for free laccase, immobilized on nanoparticles and microparticles at 54.5 degrees C, 57.5 degrees C and 46 degreesC. Dyes Direct Blue 78, Acid Blue 225, Reactive Red 195, Acid Blue 74, and Phenol Red were used for decolorization by free and immobilized laccase. Laccase was able to decolorize most of the dyes well.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Chemical and Biochemical Engineering Quarterly
ISSN
0352-9568
e-ISSN
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Svazek periodika
29
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
HR - Chorvatská republika
Počet stran výsledku
10
Strana od-do
457-466
Kód UT WoS článku
000363830800019
EID výsledku v databázi Scopus
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