Combining UV photodissociation with electron transfer for peptide structure analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A73576169" target="_blank" >RIV/61989592:15310/15:73576169 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/15:00443979

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jms.3551/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jms.3551/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jms.3551" target="_blank" >10.1002/jms.3551</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combining UV photodissociation with electron transfer for peptide structure analysis

Popis výsledku v původním jazyce

The combination of near-UV photodissociation with electron transfer and collisional activation provides a new tool for structure investigation of isolated peptide ions and reactive intermediates. Two new types of pulse experiments are reported. In the first one called UV/Vis photodissociation-electron transfer dissociation (UVPD-ETD), diazirine-labeled peptide ions are shown to undergo photodissociation in the gas phase to form new covalent bonds, guided by the ion conformation, and the products are analyzed by electron transfer dissociation. In the second experiment, called ETD-UVPD wherein synthetic labels are not necessary, electron transfer forms new cation-peptide radical chromophores that absorb at 355nm and undergo specific backbone photodissociation reactions. The new method is applied to distinguish isomeric ions produced by ETD of arginine containing peptides.

Název v anglickém jazyce

Combining UV photodissociation with electron transfer for peptide structure analysis

Popis výsledku anglicky

The combination of near-UV photodissociation with electron transfer and collisional activation provides a new tool for structure investigation of isolated peptide ions and reactive intermediates. Two new types of pulse experiments are reported. In the first one called UV/Vis photodissociation-electron transfer dissociation (UVPD-ETD), diazirine-labeled peptide ions are shown to undergo photodissociation in the gas phase to form new covalent bonds, guided by the ion conformation, and the products are analyzed by electron transfer dissociation. In the second experiment, called ETD-UVPD wherein synthetic labels are not necessary, electron transfer forms new cation-peptide radical chromophores that absorb at 355nm and undergo specific backbone photodissociation reactions. The new method is applied to distinguish isomeric ions produced by ETD of arginine containing peptides.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Mass Spectrometry

ISSN

1076-5174

e-ISSN

—

Svazek periodika

50

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

470-475

Kód UT WoS článku

000351682100006

EID výsledku v databázi Scopus

2-s2.0-84924584287