Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33160735" target="_blank" >RIV/61989592:15310/16:33160735 - isvavai.cz</a>
Výsledek na webu
<a href="http://link.springer.com/article/10.1007%2Fs11240-016-1133-z" target="_blank" >http://link.springer.com/article/10.1007%2Fs11240-016-1133-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-016-1133-z" target="_blank" >10.1007/s11240-016-1133-z</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential
Popis výsledku v původním jazyce
Beautiful vavilovia, the closest species to the common now extinct ancestor of the whole tribe Fabeae holds signiicant taxonomical interest and also for breeding within this group of species, which includes the most cultivated leguminous pulses in the world. In spite of this, vavilovia has attracted very scarce research to date and is in danger of complete extinction. Thus, as a part of the research carried out by an informal international group of researchers from various countries, we report here various experiments for the development and exploitation of a range of biotechnology tools for vavilovia, ranging from standard in vitro propagation, to plant regeneration from explant-derived callus, and also from protoplasts. Plants were successfully recovered following propagation from nodes, and by regeneration through organogenesis from callus derived from internodes (which provided the best responses) and leaves. Also, protoplasts were isolated from leaves and stems from in vitro shoots and from callus derived from these two explants, with the latter undergoing sustained division. Subsequently, protoplasts isolated from internode callus proliferated and also underwent organogenesis coupled with whole plant recovery at a low frequency, while protoplasts from leaf callus origin followed both organogenesis and embryogenesis simultaneously but failed to yield viable plants. Flow cytometry assessments permitted to ascertain the genetic idelity of both propagated and regenerated plants irrespectively of the source tissue from which they were derived (i.e., either callus from explants or from protoplasts). Finally, low cytometry also permitted us to provide the irst record on the relative nuclear DNA content and genome size for Vavilovia formosa.
Název v anglickém jazyce
Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential
Popis výsledku anglicky
Beautiful vavilovia, the closest species to the common now extinct ancestor of the whole tribe Fabeae holds signiicant taxonomical interest and also for breeding within this group of species, which includes the most cultivated leguminous pulses in the world. In spite of this, vavilovia has attracted very scarce research to date and is in danger of complete extinction. Thus, as a part of the research carried out by an informal international group of researchers from various countries, we report here various experiments for the development and exploitation of a range of biotechnology tools for vavilovia, ranging from standard in vitro propagation, to plant regeneration from explant-derived callus, and also from protoplasts. Plants were successfully recovered following propagation from nodes, and by regeneration through organogenesis from callus derived from internodes (which provided the best responses) and leaves. Also, protoplasts were isolated from leaves and stems from in vitro shoots and from callus derived from these two explants, with the latter undergoing sustained division. Subsequently, protoplasts isolated from internode callus proliferated and also underwent organogenesis coupled with whole plant recovery at a low frequency, while protoplasts from leaf callus origin followed both organogenesis and embryogenesis simultaneously but failed to yield viable plants. Flow cytometry assessments permitted to ascertain the genetic idelity of both propagated and regenerated plants irrespectively of the source tissue from which they were derived (i.e., either callus from explants or from protoplasts). Finally, low cytometry also permitted us to provide the irst record on the relative nuclear DNA content and genome size for Vavilovia formosa.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EF - Botanika
OECD FORD obor
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Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plant Cell, Tissue and Organ Culture (print)
ISSN
0167-6857
e-ISSN
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Svazek periodika
127
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
12
Strana od-do
"637-648"
Kód UT WoS článku
000391389700011
EID výsledku v databázi Scopus
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