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Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33160735" target="_blank" >RIV/61989592:15310/16:33160735 - isvavai.cz</a>

  • Výsledek na webu

    <a href="http://link.springer.com/article/10.1007%2Fs11240-016-1133-z" target="_blank" >http://link.springer.com/article/10.1007%2Fs11240-016-1133-z</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11240-016-1133-z" target="_blank" >10.1007/s11240-016-1133-z</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential

  • Popis výsledku v původním jazyce

    Beautiful vavilovia, the closest species to the common now extinct ancestor of the whole tribe Fabeae holds signiicant taxonomical interest and also for breeding within this group of species, which includes the most cultivated leguminous pulses in the world. In spite of this, vavilovia has attracted very scarce research to date and is in danger of complete extinction. Thus, as a part of the research carried out by an informal international group of researchers from various countries, we report here various experiments for the development and exploitation of a range of biotechnology tools for vavilovia, ranging from standard in vitro propagation, to plant regeneration from explant-derived callus, and also from protoplasts. Plants were successfully recovered following propagation from nodes, and by regeneration through organogenesis from callus derived from internodes (which provided the best responses) and leaves. Also, protoplasts were isolated from leaves and stems from in vitro shoots and from callus derived from these two explants, with the latter undergoing sustained division. Subsequently, protoplasts isolated from internode callus proliferated and also underwent organogenesis coupled with whole plant recovery at a low frequency, while protoplasts from leaf callus origin followed both organogenesis and embryogenesis simultaneously but failed to yield viable plants. Flow cytometry assessments permitted to ascertain the genetic idelity of both propagated and regenerated plants irrespectively of the source tissue from which they were derived (i.e., either callus from explants or from protoplasts). Finally, low cytometry also permitted us to provide the irst record on the relative nuclear DNA content and genome size for Vavilovia formosa.

  • Název v anglickém jazyce

    Developing biotechnology tools for 'beautiful' vavilovia (Vavilovia formosa), a legume crop wild relative with taxonomic and agronomic potential

  • Popis výsledku anglicky

    Beautiful vavilovia, the closest species to the common now extinct ancestor of the whole tribe Fabeae holds signiicant taxonomical interest and also for breeding within this group of species, which includes the most cultivated leguminous pulses in the world. In spite of this, vavilovia has attracted very scarce research to date and is in danger of complete extinction. Thus, as a part of the research carried out by an informal international group of researchers from various countries, we report here various experiments for the development and exploitation of a range of biotechnology tools for vavilovia, ranging from standard in vitro propagation, to plant regeneration from explant-derived callus, and also from protoplasts. Plants were successfully recovered following propagation from nodes, and by regeneration through organogenesis from callus derived from internodes (which provided the best responses) and leaves. Also, protoplasts were isolated from leaves and stems from in vitro shoots and from callus derived from these two explants, with the latter undergoing sustained division. Subsequently, protoplasts isolated from internode callus proliferated and also underwent organogenesis coupled with whole plant recovery at a low frequency, while protoplasts from leaf callus origin followed both organogenesis and embryogenesis simultaneously but failed to yield viable plants. Flow cytometry assessments permitted to ascertain the genetic idelity of both propagated and regenerated plants irrespectively of the source tissue from which they were derived (i.e., either callus from explants or from protoplasts). Finally, low cytometry also permitted us to provide the irst record on the relative nuclear DNA content and genome size for Vavilovia formosa.

Klasifikace

  • Druh

    J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

  • CEP obor

    EF - Botanika

  • OECD FORD obor

Návaznosti výsledku

  • Projekt

  • Návaznosti

    S - Specificky vyzkum na vysokych skolach

Ostatní

  • Rok uplatnění

    2016

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Plant Cell, Tissue and Organ Culture (print)

  • ISSN

    0167-6857

  • e-ISSN

  • Svazek periodika

    127

  • Číslo periodika v rámci svazku

    3

  • Stát vydavatele periodika

    NL - Nizozemsko

  • Počet stran výsledku

    12

  • Strana od-do

    "637-648"

  • Kód UT WoS článku

    000391389700011

  • EID výsledku v databázi Scopus