Bcl-2421 and Ac-DEVD-CHO Inhibit Death of Wheat Microspores

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33161328" target="_blank" >RIV/61989592:15310/16:33161328 - isvavai.cz</a>

Výsledek na webu

<a href="http://journal.frontiersin.org/article/10.3389/fpls.2016.01931/full" target="_blank" >http://journal.frontiersin.org/article/10.3389/fpls.2016.01931/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2016.01931" target="_blank" >10.3389/fpls.2016.01931</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bcl-2421 and Ac-DEVD-CHO Inhibit Death of Wheat Microspores

Popis výsledku v původním jazyce

Microspore cell death and low green plant production efficiency are an integral obstacle in the development of doubled haploid production in wheat. The aim of the current study was to determine the effect of anti-apoptotic recombinant human B-cell lymphoma-2 (Bcl-2421) and caspase-3-inhibitor (Ac-DEVD-CHO) in microspore cell death in bread wheat cultivars AC Fielder and AC Andrew. Induction medium containing Bcl-2421 and Ac-DEVD-CHO yielded a significantly higher number of viable microspores, embryo-like structures and total green plants in wheat cultivars AC Fielder and AC Andrew. Total peroxidase activity was lower in Bcl-2421 treated microspore cultures at 96 h of treatment compared to control and Ac-DEVD-CHO. Electron paramagnetic resonance study of total microspore protein showed a different scavenging aktivity for Bcl-2421 and Ac-DEVD-CHO. Bcl-2421 scavenged approximately 50% hydroxyl radical (HO) formed, whereas Ac-DEVD-CHO scavenged approximately 20% of HO. Conversely, reduced caspase-3-like activities were detected in the presence of Bcl-2421 and Ac-DEVD-CHO, supporting the involvement of Bcl-2421 and Ac-DEVD-CHO in increasing microspore viability by reducing oxidative stress and caspase-3-like activity. Our results indicate that Bcl-2421 and Ac-DEVD-CHO protects cells from cell death following different pathways. Bcl-2421 prevents cell damage by detoxifying HO and suppressing caspase-3-like activity, while Ac-DEVD-CHO inhibits the cell death pathways by modulating caspase-like activity.

Název v anglickém jazyce

Bcl-2421 and Ac-DEVD-CHO Inhibit Death of Wheat Microspores

Popis výsledku anglicky

Microspore cell death and low green plant production efficiency are an integral obstacle in the development of doubled haploid production in wheat. The aim of the current study was to determine the effect of anti-apoptotic recombinant human B-cell lymphoma-2 (Bcl-2421) and caspase-3-inhibitor (Ac-DEVD-CHO) in microspore cell death in bread wheat cultivars AC Fielder and AC Andrew. Induction medium containing Bcl-2421 and Ac-DEVD-CHO yielded a significantly higher number of viable microspores, embryo-like structures and total green plants in wheat cultivars AC Fielder and AC Andrew. Total peroxidase activity was lower in Bcl-2421 treated microspore cultures at 96 h of treatment compared to control and Ac-DEVD-CHO. Electron paramagnetic resonance study of total microspore protein showed a different scavenging aktivity for Bcl-2421 and Ac-DEVD-CHO. Bcl-2421 scavenged approximately 50% hydroxyl radical (HO) formed, whereas Ac-DEVD-CHO scavenged approximately 20% of HO. Conversely, reduced caspase-3-like activities were detected in the presence of Bcl-2421 and Ac-DEVD-CHO, supporting the involvement of Bcl-2421 and Ac-DEVD-CHO in increasing microspore viability by reducing oxidative stress and caspase-3-like activity. Our results indicate that Bcl-2421 and Ac-DEVD-CHO protects cells from cell death following different pathways. Bcl-2421 prevents cell damage by detoxifying HO and suppressing caspase-3-like activity, while Ac-DEVD-CHO inhibits the cell death pathways by modulating caspase-like activity.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/LO1204" target="_blank" >LO1204: Udržitelný rozvoj výzkumu v Centru regionu Haná</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

1931

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

"1931-1"-"1931-12"

Kód UT WoS článku

000390595000001

EID výsledku v databázi Scopus

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