A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2 '-deoxyguanosinein human urine and plasma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33161345" target="_blank" >RIV/61989592:15310/16:33161345 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/16:33161345 RIV/00216224:14310/16:00091051 RIV/70883521:28110/16:43874638

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0003267016308078" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0003267016308078</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2016.06.044" target="_blank" >10.1016/j.aca.2016.06.044</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2 '-deoxyguanosinein human urine and plasma

Popis výsledku v původním jazyce

A novel method of carbon fiber microelectrode activation using spark discharge was demonstrated and compared to conventional electrochemical pretreatment by potential cycling. The spark discharge was performed at 800 V between the microelectrode connected to positive pole of the power supply and platinum counter electrode. Spark discharge led both to trimming of the fiber tip into conical shape and to the modification of carbon fiber microelectrode with platinum, as proven by scanning electron microscopy and electron dispersive X-ray spectroscopy. After the characterization of electrochemical properties using ferricyanide voltammetry, the activated electrodes were used for electrochemical analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an oxidative stress marker. Subnanomolar detection limits (0.55 nmol L-1) in high-performance liquid chromatography were achieved for spark platinized electrodes incorporated into the flow detection cell.

Název v anglickém jazyce

A comparison of electrochemically pre-treated and spark-platinized carbon fiber microelectrode. Measurement of 8-oxo-7,8-dihydro-2 '-deoxyguanosinein human urine and plasma

Popis výsledku anglicky

A novel method of carbon fiber microelectrode activation using spark discharge was demonstrated and compared to conventional electrochemical pretreatment by potential cycling. The spark discharge was performed at 800 V between the microelectrode connected to positive pole of the power supply and platinum counter electrode. Spark discharge led both to trimming of the fiber tip into conical shape and to the modification of carbon fiber microelectrode with platinum, as proven by scanning electron microscopy and electron dispersive X-ray spectroscopy. After the characterization of electrochemical properties using ferricyanide voltammetry, the activated electrodes were used for electrochemical analysis of 8-oxo-7,8-dihydro-2'-deoxyguanosine, an oxidative stress marker. Subnanomolar detection limits (0.55 nmol L-1) in high-performance liquid chromatography were achieved for spark platinized electrodes incorporated into the flow detection cell.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA15-05198S" target="_blank" >GA15-05198S: Nanostrukturované kovové vrstvy na vodivých a polovodivých substrátech pro aplikace v chemické a biomedicínské analýze</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

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Svazek periodika

935

Číslo periodika v rámci svazku

SEP

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

82-89

Kód UT WoS článku

000382249600005

EID výsledku v databázi Scopus

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