Advances in Imaging Plant Cell Dynamics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73589791" target="_blank" >RIV/61989592:15310/18:73589791 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.plantphysiol.org/content/176/1/80" target="_blank" >http://www.plantphysiol.org/content/176/1/80</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1104/pp.17.00962" target="_blank" >10.1104/pp.17.00962</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Advances in Imaging Plant Cell Dynamics

Popis výsledku v původním jazyce

After the establishment of advanced fluorescence microscopy methods and the development of numerous fluorescent proteins, it is possible to follow the organization and dynamics of most organelles and subcellular compartments in cells of living plants. Nowadays, it is possible to address subcellular architecture at the nanoscale through the implementation of superresolution microscopy methods such as structured illumination microscopy, photoactivation localization microscopy, or stochastic optical reconstruction and stimulated emission depletion microscopy. In a developmental context, the dynamic cellular and subcellular changes can be monitored long term in whole plant organs by light-sheet fluorescence microscopy. This is a mesoscopic method offering high-speed imaging, very low phototoxicity, and bioimaging of vertically oriented plants. This Update aims to provide the principles, the current application range, and the expected potential of superresolution and light-sheet fluorescence microscopy methods as well as a brief description of the improvements of standard wide-field epifluorescence and confocal systems.

Název v anglickém jazyce

Advances in Imaging Plant Cell Dynamics

Popis výsledku anglicky

After the establishment of advanced fluorescence microscopy methods and the development of numerous fluorescent proteins, it is possible to follow the organization and dynamics of most organelles and subcellular compartments in cells of living plants. Nowadays, it is possible to address subcellular architecture at the nanoscale through the implementation of superresolution microscopy methods such as structured illumination microscopy, photoactivation localization microscopy, or stochastic optical reconstruction and stimulated emission depletion microscopy. In a developmental context, the dynamic cellular and subcellular changes can be monitored long term in whole plant organs by light-sheet fluorescence microscopy. This is a mesoscopic method offering high-speed imaging, very low phototoxicity, and bioimaging of vertically oriented plants. This Update aims to provide the principles, the current application range, and the expected potential of superresolution and light-sheet fluorescence microscopy methods as well as a brief description of the improvements of standard wide-field epifluorescence and confocal systems.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT PHYSIOLOGY

ISSN

0032-0889

e-ISSN

—

Svazek periodika

176

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

80-93

Kód UT WoS článku

000419675300006

EID výsledku v databázi Scopus

2-s2.0-85040662736