Chemical quenching of singlet oxygen by plastoquinols and their oxidation products in Arabidopsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73590093" target="_blank" >RIV/61989592:15310/18:73590093 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13993" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13993</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/tpj.13993" target="_blank" >10.1111/tpj.13993</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chemical quenching of singlet oxygen by plastoquinols and their oxidation products in Arabidopsis

Popis výsledku v původním jazyce

Prenylquinols (tocochromanols and plastoquinols) serve as efficient physical and chemical quenchers of singlet oxygen (1O2) formed during high light stress in higher plants. Although quenching of 1O2 by prenylquinols has been previously studied, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is limited in vivo. In the present study, the role of plastoquinol-9 (PQH2-9) in chemical quenching of 1O2 was studied in Arabidopsis thaliana lines overexpressing the SOLANESYL DIPHOSPHATE SYNTHASE 1 gene (SPS1oex) involved in PQH2-9 and plastochromanol-8 biosynthesis. In this work, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is presented, which is obtained by microscopic techniques in vivo. Chemical quenching of 1O2 was associated with consumption of PQH2-9 and formation of its various oxidized forms. Oxidation of PQH2-9 by 1O2 leads to plastoquinone-9 (PQ-9), which is subsequently oxidized to hydroxyplastoquinone-9 [PQ(OH)-9]. We provide here evidence that oxidation of PQ(OH)-9 by 1O2 results in the formation of trihydroxyplastoquinone-9 [PQ(OH)3-9]. It is concluded here that PQH2-9 serves as an efficient 1O2 chemical quencher in Arabidopsis, and PQ(OH)3-9 can be considered as a natural product of 1O2 reaction with PQ(OH)-9. The understanding of the mechanisms underlying 1O2 chemical quenching provides information on the role of plastoquinols and their oxidation products in the response of plants to photooxidative stress.

Název v anglickém jazyce

Chemical quenching of singlet oxygen by plastoquinols and their oxidation products in Arabidopsis

Popis výsledku anglicky

Prenylquinols (tocochromanols and plastoquinols) serve as efficient physical and chemical quenchers of singlet oxygen (1O2) formed during high light stress in higher plants. Although quenching of 1O2 by prenylquinols has been previously studied, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is limited in vivo. In the present study, the role of plastoquinol-9 (PQH2-9) in chemical quenching of 1O2 was studied in Arabidopsis thaliana lines overexpressing the SOLANESYL DIPHOSPHATE SYNTHASE 1 gene (SPS1oex) involved in PQH2-9 and plastochromanol-8 biosynthesis. In this work, direct evidence for chemical quenching of 1O2 by plastoquinols and their oxidation products is presented, which is obtained by microscopic techniques in vivo. Chemical quenching of 1O2 was associated with consumption of PQH2-9 and formation of its various oxidized forms. Oxidation of PQH2-9 by 1O2 leads to plastoquinone-9 (PQ-9), which is subsequently oxidized to hydroxyplastoquinone-9 [PQ(OH)-9]. We provide here evidence that oxidation of PQ(OH)-9 by 1O2 results in the formation of trihydroxyplastoquinone-9 [PQ(OH)3-9]. It is concluded here that PQH2-9 serves as an efficient 1O2 chemical quencher in Arabidopsis, and PQ(OH)3-9 can be considered as a natural product of 1O2 reaction with PQ(OH)-9. The understanding of the mechanisms underlying 1O2 chemical quenching provides information on the role of plastoquinols and their oxidation products in the response of plants to photooxidative stress.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/LO1204" target="_blank" >LO1204: Udržitelný rozvoj výzkumu v Centru regionu Haná</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLANT JOURNAL

ISSN

0960-7412

e-ISSN

—

Svazek periodika

95

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

"848–861"

Kód UT WoS článku

000441759400009

EID výsledku v databázi Scopus

2-s2.0-85051487358