Half-Sandwich Ru(II) and Os(II) Bathophenanthroline Complexes Containing a Releasable Dichloroacetato Ligand

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73591072" target="_blank" >RIV/61989592:15310/18:73591072 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/1420-3049/23/2/420/htm" target="_blank" >https://www.mdpi.com/1420-3049/23/2/420/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules23020420" target="_blank" >10.3390/molecules23020420</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Half-Sandwich Ru(II) and Os(II) Bathophenanthroline Complexes Containing a Releasable Dichloroacetato Ligand

Popis výsledku v původním jazyce

We report on the preparation and thorough characterization of cytotoxic half-sandwich complexes [Ru(eta(6)-pcym)(bphen)(dca)]PF6 (Ru-dca) and [Os(eta(6)-pcym)(bphen)(dca)]PF6 (Os-dca) containing dichloroacetate(1-) (dca) as the releasable O-donor ligand bearing its own cytotoxicity; pcym = 1-methyl-4-(propan-2-yl) benzene (p-cymene), bphen = 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline). Complexes Ru-dca and Os-dca hydrolyzed in the water-containing media, which led to the dca ligand release (supported by H-1 NMR and electrospray ionization mass spectra). Mass spectrometry studies revealed that complexes Ru-dca and Os-dca do not interact covalently with the model proteins cytochrome c and lysozyme. Both complexes exhibited slightly higher in vitro cytotoxicity (IC50 = 3.5 mu M for Ru-dca, and 2.6 mu M for Os-dca) against the A2780 human ovarian carcinoma cells than cisplatin (IC50 = 5.9 mu M), while their toxicity on the healthy human hepatocytes was found to be IC50 = 19.1 mu M for Ru-dca and IC50 = 19.7 mu M for Os-dca. Despite comparable cytotoxicity of complexes Ru-dca and Os-dca, both the complexes modified the cell cycle, mitochondrial membrane potential, and mitochondrial cytochrome c release by a different way, as revealed by flow cytometry experiments. The obtained results point out the different mechanisms of action between the complexes.

Název v anglickém jazyce

Half-Sandwich Ru(II) and Os(II) Bathophenanthroline Complexes Containing a Releasable Dichloroacetato Ligand

Popis výsledku anglicky

We report on the preparation and thorough characterization of cytotoxic half-sandwich complexes [Ru(eta(6)-pcym)(bphen)(dca)]PF6 (Ru-dca) and [Os(eta(6)-pcym)(bphen)(dca)]PF6 (Os-dca) containing dichloroacetate(1-) (dca) as the releasable O-donor ligand bearing its own cytotoxicity; pcym = 1-methyl-4-(propan-2-yl) benzene (p-cymene), bphen = 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline). Complexes Ru-dca and Os-dca hydrolyzed in the water-containing media, which led to the dca ligand release (supported by H-1 NMR and electrospray ionization mass spectra). Mass spectrometry studies revealed that complexes Ru-dca and Os-dca do not interact covalently with the model proteins cytochrome c and lysozyme. Both complexes exhibited slightly higher in vitro cytotoxicity (IC50 = 3.5 mu M for Ru-dca, and 2.6 mu M for Os-dca) against the A2780 human ovarian carcinoma cells than cisplatin (IC50 = 5.9 mu M), while their toxicity on the healthy human hepatocytes was found to be IC50 = 19.1 mu M for Ru-dca and IC50 = 19.7 mu M for Os-dca. Despite comparable cytotoxicity of complexes Ru-dca and Os-dca, both the complexes modified the cell cycle, mitochondrial membrane potential, and mitochondrial cytochrome c release by a different way, as revealed by flow cytometry experiments. The obtained results point out the different mechanisms of action between the complexes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MOLECULES

ISSN

1420-3049

e-ISSN

—

Svazek periodika

23

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

16

Strana od-do

"420-1"-"420-16"

Kód UT WoS článku

000426436300200

EID výsledku v databázi Scopus

2-s2.0-85042228130