Effects of Flavored Nonalcoholic Beverages on Transcriptional Activities of Nuclear and Steroid Hormone Receptors: Proof of Concept for Novel Reporter Cell Line PAZ-PPARg

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73591319" target="_blank" >RIV/61989592:15310/18:73591319 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acs.jafc.8b05158" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jafc.8b05158</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.jafc.8b05158" target="_blank" >10.1021/acs.jafc.8b05158</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of Flavored Nonalcoholic Beverages on Transcriptional Activities of Nuclear and Steroid Hormone Receptors: Proof of Concept for Novel Reporter Cell Line PAZ-PPARg

Popis výsledku v původním jazyce

We developed and characterized a novel human luciferase reporter cell line for the assessment of peroxisome proliferator-activated receptor gamma (PPAR gamma) transcriptional activity, PAZ-PPARg. The luciferase activity induced by PPAR gamma endogenous agonist 15d-PGJ2 and prostaglandin PGD2 reached mean values of (87.9 +/- 14.0)-fold and (89.6 +/- 19.7)-fold after 24 h of exposure to 40 mu M 15d-PGJ2 and 70 mu M PGD2, respectively. A concentration-dependent inhibition of 15d-PGJ2- and PGD2-induced luciferase activity was observed after the application of T0070907, a selective antagonist of PPAR gamma, which confirms the specificity of response to both agonists. The PAZ-PPARg cell line, along with the reporter cell lines for the assessment of transcriptional activities of thyroid receptor (TR), vitamin D3 receptor (VDR), androgen receptor (AR), and glucocorticoid receptor (GR), were used for the screening of 27 commonly marketed flavored nonalcoholic beverages for their possible disrupting effects. Our findings indicate that some of the examined beverages have the potential to modulate the transcriptional activities of PPAR gamma, VDR, and AR.

Název v anglickém jazyce

Effects of Flavored Nonalcoholic Beverages on Transcriptional Activities of Nuclear and Steroid Hormone Receptors: Proof of Concept for Novel Reporter Cell Line PAZ-PPARg

Popis výsledku anglicky

We developed and characterized a novel human luciferase reporter cell line for the assessment of peroxisome proliferator-activated receptor gamma (PPAR gamma) transcriptional activity, PAZ-PPARg. The luciferase activity induced by PPAR gamma endogenous agonist 15d-PGJ2 and prostaglandin PGD2 reached mean values of (87.9 +/- 14.0)-fold and (89.6 +/- 19.7)-fold after 24 h of exposure to 40 mu M 15d-PGJ2 and 70 mu M PGD2, respectively. A concentration-dependent inhibition of 15d-PGJ2- and PGD2-induced luciferase activity was observed after the application of T0070907, a selective antagonist of PPAR gamma, which confirms the specificity of response to both agonists. The PAZ-PPARg cell line, along with the reporter cell lines for the assessment of transcriptional activities of thyroid receptor (TR), vitamin D3 receptor (VDR), androgen receptor (AR), and glucocorticoid receptor (GR), were used for the screening of 27 commonly marketed flavored nonalcoholic beverages for their possible disrupting effects. Our findings indicate that some of the examined beverages have the potential to modulate the transcriptional activities of PPAR gamma, VDR, and AR.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN

0021-8561

e-ISSN

—

Svazek periodika

66

Číslo periodika v rámci svazku

45

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

12066-12078

Kód UT WoS článku

000451102000021

EID výsledku v databázi Scopus

2-s2.0-85056322715