Intrinsic photoluminescence of amine-functionalized graphene derivatives for bioimaging applications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73597856" target="_blank" >RIV/61989592:15310/19:73597856 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2352940719305633" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2352940719305633</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apmt.2019.08.002" target="_blank" >10.1016/j.apmt.2019.08.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Intrinsic photoluminescence of amine-functionalized graphene derivatives for bioimaging applications

Popis výsledku v původním jazyce

Photoluminescent graphene-based materials have enormous application potential in cell imaging, display technologies, biomedicine and biosensing. Therefore, their development represents a principal yet highly challenging task for graphene chemistry. Up to now, strategies based on the size confinement in graphene/graphene oxide (GO) quantum dots, non-covalent chemistry combining GO with photoluminescence species, and GO chemistry enabling band gap tuning have been reported. Here, we introduce a simple approach to intrinsically photoluminescent graphene derivatives via one-step fluorographene chemistry enabling controlled surface engineering/chemical reduction by amines. Specifically, the reaction of fluorographene with dodecylamine and hexamethylenediamine results in organophilic and hydrophilic graphene derivatives, respectively, exhibiting intrinsic fluorescence. Both density functional theory calculations and experimental data show that the emission properties occur because of the energy gaps engineered by the choice of amine. Cytotoxicity measurements on NIH/3T3 and HeLa cells demonstrated high biocompatibility for the hydrophilic amine-functionalized derivative. Due to the intrinsic fluorescence, quantification of the uptake by cells and localization of graphene-based sheets in cells can be performed directly using a flow cytometry technique and fluorescence microscopy imaging. These findings pave the way for a new class of functional photoluminescent graphene derivatives with high application potential in fields like biosensing, biomedicine and bioimaging.

Název v anglickém jazyce

Intrinsic photoluminescence of amine-functionalized graphene derivatives for bioimaging applications

Popis výsledku anglicky

Photoluminescent graphene-based materials have enormous application potential in cell imaging, display technologies, biomedicine and biosensing. Therefore, their development represents a principal yet highly challenging task for graphene chemistry. Up to now, strategies based on the size confinement in graphene/graphene oxide (GO) quantum dots, non-covalent chemistry combining GO with photoluminescence species, and GO chemistry enabling band gap tuning have been reported. Here, we introduce a simple approach to intrinsically photoluminescent graphene derivatives via one-step fluorographene chemistry enabling controlled surface engineering/chemical reduction by amines. Specifically, the reaction of fluorographene with dodecylamine and hexamethylenediamine results in organophilic and hydrophilic graphene derivatives, respectively, exhibiting intrinsic fluorescence. Both density functional theory calculations and experimental data show that the emission properties occur because of the energy gaps engineered by the choice of amine. Cytotoxicity measurements on NIH/3T3 and HeLa cells demonstrated high biocompatibility for the hydrophilic amine-functionalized derivative. Due to the intrinsic fluorescence, quantification of the uptake by cells and localization of graphene-based sheets in cells can be performed directly using a flow cytometry technique and fluorescence microscopy imaging. These findings pave the way for a new class of functional photoluminescent graphene derivatives with high application potential in fields like biosensing, biomedicine and bioimaging.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Materials Today

ISSN

2352-9407

e-ISSN

—

Svazek periodika

17

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

112-122

Kód UT WoS článku

000502851600007

EID výsledku v databázi Scopus

2-s2.0-85070708988