Phospholipid-modified carbon fiber brush electrode for the detection of dopamine and 3,4-dihydroxyphenylacetic acid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F19%3A73597894" target="_blank" >RIV/61989592:15310/19:73597894 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs00706-019-2371-7" target="_blank" >https://link.springer.com/article/10.1007%2Fs00706-019-2371-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-019-2371-7" target="_blank" >10.1007/s00706-019-2371-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phospholipid-modified carbon fiber brush electrode for the detection of dopamine and 3,4-dihydroxyphenylacetic acid

Popis výsledku v původním jazyce

Different procedures for modification of carbon fiber brush electrode (CFBE) with phospholipids were investigated using cyclic voltammetry and electrochemical impedance spectroscopy. Simple immersion of the preanodized electrode into hexane solution of asolectin (a mixture of soybean phospholipids) followed by formation of the phospholipid layer in NaCl solution was the most effective procedure. Phospholipid layer-modified electrode (PL-CFBE) was applied to detect dopamine and its metabolite, 3,4 dihydroxyphenylacetic acid, using differential pulse and square-wave voltammetry in phosphate buffer solution of pH 6.5. Accumulation of dopamine, supported by electrostatic interactions between positively charged amino group and negatively charged phosphate moiety of the layer, enhanced sensitivity of PL-CFBE compared to bare CFBE. On the contrary, repulsive interaction between 3,4 dihydroxyphenylacetate anion and negatively charged phosphate moiety of the layer suppressed PL-CFBE sensitivity, measured as a slope of calibration dependence.

Název v anglickém jazyce

Phospholipid-modified carbon fiber brush electrode for the detection of dopamine and 3,4-dihydroxyphenylacetic acid

Popis výsledku anglicky

Different procedures for modification of carbon fiber brush electrode (CFBE) with phospholipids were investigated using cyclic voltammetry and electrochemical impedance spectroscopy. Simple immersion of the preanodized electrode into hexane solution of asolectin (a mixture of soybean phospholipids) followed by formation of the phospholipid layer in NaCl solution was the most effective procedure. Phospholipid layer-modified electrode (PL-CFBE) was applied to detect dopamine and its metabolite, 3,4 dihydroxyphenylacetic acid, using differential pulse and square-wave voltammetry in phosphate buffer solution of pH 6.5. Accumulation of dopamine, supported by electrostatic interactions between positively charged amino group and negatively charged phosphate moiety of the layer, enhanced sensitivity of PL-CFBE compared to bare CFBE. On the contrary, repulsive interaction between 3,4 dihydroxyphenylacetate anion and negatively charged phosphate moiety of the layer suppressed PL-CFBE sensitivity, measured as a slope of calibration dependence.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA17-05387S" target="_blank" >GA17-05387S: Nové přístupy pro sledování přenosu zvolených xenobiotik přes biomimetické membrány</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MONATSHEFTE FUR CHEMIE

ISSN

0026-9247

e-ISSN

—

Svazek periodika

150

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

6

Strana od-do

395-400

Kód UT WoS článku

000461394400005

EID výsledku v databázi Scopus

2-s2.0-85061108088