Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized beta-cyclodextrins as mobile-phase additive

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73603970" target="_blank" >RIV/61989592:15310/20:73603970 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.202000268" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.202000268</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202000268" target="_blank" >10.1002/jssc.202000268</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized beta-cyclodextrins as mobile-phase additive

Popis výsledku v původním jazyce

A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin. The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-beta-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-beta-cyclodextrin. The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated. At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-beta-cyclodextrin and 10 mM of sulfobutyl ether-beta-cyclodextrin, respectively. Precision, linearity, and sensitivity of the method were tested. Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 mu g/mL, respectively. The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides inCitrusjuices after solid-phase extraction procedure.

Název v anglickém jazyce

Stereoisomer separation of flavanones and flavanone-7-O-glycosides by means of nanoliquid chromatography employing derivatized beta-cyclodextrins as mobile-phase additive

Popis výsledku anglicky

A nanoliquid chromatographic method for the stereoisomer separation of some flavanone aglycones and 7-O-glycosides has been proposed employing a C18 capillary column and a chiral mobile-phase additive such as cyclodextrin. The chiral separation of eriodictyol, naringenin, and hesperitin was obtained by addition of carboxymethyl-beta-cyclodextrin to the mobile phase, whereas eriocitrin, naringin, narirutin, and hesperidin diastereoisomers were resolved by using sulfobutyl ether-beta-cyclodextrin. The influence of the composition of the mobile phase, the length of the capillary column, and the flow rate on the chiral recognition were investigated. At optimum conditions, baseline separation for the selected aglycones and glycosylated forms were achieved with a mobile phase consisting of 50 mM sodium acetate buffer pH 3 and 30% methanol containing 20 mM of carboxymethyl-beta-cyclodextrin and 10 mM of sulfobutyl ether-beta-cyclodextrin, respectively. Precision, linearity, and sensitivity of the method were tested. Limits of detection and quantification for the studied flavanone glycosides were in the range 1.3-2.5 and 7.5-12.5 mu g/mL, respectively. The method was used for the determination of the diastereomeric composition of the flavanone-7-O-glycosides inCitrusjuices after solid-phase extraction procedure.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/EF16_019%2F0000754" target="_blank" >EF16_019/0000754: Nanotechnologie pro budoucnost</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF SEPARATION SCIENCE

ISSN

1615-9306

e-ISSN

—

Svazek periodika

43

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

3382-3390

Kód UT WoS článku

000557665400001

EID výsledku v databázi Scopus

2-s2.0-85089159720