PSI of the Colonial Alga Botryococcus braunii Has an Unusually Large Antenna Size
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73604420" target="_blank" >RIV/61989592:15310/20:73604420 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.plantphysiol.org/content/plantphysiol/184/4/2040.full.pdf" target="_blank" >http://www.plantphysiol.org/content/plantphysiol/184/4/2040.full.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1104/pp.20.00823" target="_blank" >10.1104/pp.20.00823</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
PSI of the Colonial Alga Botryococcus braunii Has an Unusually Large Antenna Size
Popis výsledku v původním jazyce
PSI is an essential component of the photosynthetic apparatus of oxygenic photosynthesis. While most of its subunits are conserved, recent data have shown that the arrangement of the light-harvesting complexes I (LHCIs) differs substantially in different organisms. Here we studied the PSI-LHCI supercomplex of Botryococccus braunii, a colonial green alga with potential for lipid and sugar production, using functional analysis and single-particle electron microscopy of the isolated PSI-LHCI supercomplexes complemented by time-resolved fluorescence spectroscopy in vivo. We established that the largest purified PSI-LHCI supercomplex contains 10 LHCIs (;240 chlorophylls). However, electron microscopy showed heterogeneity in the particles and a total of 13 unique binding sites for the LHCIs around the PSI core. Time-resolved fluorescence spectroscopy indicated that the PSI antenna size in vivo is even larger than that of the purified complex. Based on the comparison of the known PSI structures, we propose that PSI in B. braunii can bind LHCIs at all known positions surrounding the core. This organization maximizes the antenna size while maintaining fast excitation energy transfer, and thus high trapping efficiency, within the complex.
Název v anglickém jazyce
PSI of the Colonial Alga Botryococcus braunii Has an Unusually Large Antenna Size
Popis výsledku anglicky
PSI is an essential component of the photosynthetic apparatus of oxygenic photosynthesis. While most of its subunits are conserved, recent data have shown that the arrangement of the light-harvesting complexes I (LHCIs) differs substantially in different organisms. Here we studied the PSI-LHCI supercomplex of Botryococccus braunii, a colonial green alga with potential for lipid and sugar production, using functional analysis and single-particle electron microscopy of the isolated PSI-LHCI supercomplexes complemented by time-resolved fluorescence spectroscopy in vivo. We established that the largest purified PSI-LHCI supercomplex contains 10 LHCIs (;240 chlorophylls). However, electron microscopy showed heterogeneity in the particles and a total of 13 unique binding sites for the LHCIs around the PSI core. Time-resolved fluorescence spectroscopy indicated that the PSI antenna size in vivo is even larger than that of the purified complex. Based on the comparison of the known PSI structures, we propose that PSI in B. braunii can bind LHCIs at all known positions surrounding the core. This organization maximizes the antenna size while maintaining fast excitation energy transfer, and thus high trapping efficiency, within the complex.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10610 - Biophysics
Návaznosti výsledku
Projekt
<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
PLANT PHYSIOLOGY
ISSN
0032-0889
e-ISSN
—
Svazek periodika
2020
Číslo periodika v rámci svazku
184
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
12
Strana od-do
2040-2051
Kód UT WoS článku
000601303200037
EID výsledku v databázi Scopus
2-s2.0-85097495242