Morphological and genetic diversity of Slovene lettuce landrace ‘Ljubljanska ledenka’ (Lactuca sativa L.)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73602484" target="_blank" >RIV/61989592:15310/21:73602484 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10722-020-00978-5" target="_blank" >https://link.springer.com/article/10.1007/s10722-020-00978-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10722-020-00978-5" target="_blank" >10.1007/s10722-020-00978-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Morphological and genetic diversity of Slovene lettuce landrace ‘Ljubljanska ledenka’ (Lactuca sativa L.)

Popis výsledku v původním jazyce

A set of accessions of lettuce landrace ‘Ljubljanska ledenka’ (Lactuca sativa L.) was characterized by morphological and molecular markers and for resistance to Bremia lactucae, with the aim of assessing the variability of the collection, exploring the genetic structure and potentially identifying the characters responsible for differentiation of the accessions. Wide phenotypic variation was observed among 51 accessions screened for 26 morphological and phenological traits. UPGMA cluster analysis and principal component analysis based on phenotypic data enabled the studied accessions to be divided into four clusters. The most important character by which the largest two clusters were differentiated was anthocyanin coloration. The clustering pattern of the AFLP dendrogram where two major clusters were identified was similar but not identical to the pattern of the phenotypic dendrogram. The Mantel test showed a high correlation between the phenotypic and the molecular data obtained (r = 0.67). In spite of the weak genetic differentiation between the accessions, STRUCTURE analysis based on AFLP data provided a clear indication for the existence of sub-groups within the two clusters. Thirty-four accessions were screened for resistance to 12 races of B. lactucae. The results show that the accessions very frequently express various reaction patterns of race specificity. Expression of race-specificity was not uniform across the set of accessions and at least 11 different reaction patterns were recorded, indicating that different racespecific resistance factors (R-factors) or genes (Dm genes) could be expected. This conclusion is supported by the results obtained in morphological and molecular studies, showing that lettuce landrace ‘Ljubljanska ledenka’ is not genetically uniform and is represented by a variety of genotypes.

Název v anglickém jazyce

Morphological and genetic diversity of Slovene lettuce landrace ‘Ljubljanska ledenka’ (Lactuca sativa L.)

Popis výsledku anglicky

A set of accessions of lettuce landrace ‘Ljubljanska ledenka’ (Lactuca sativa L.) was characterized by morphological and molecular markers and for resistance to Bremia lactucae, with the aim of assessing the variability of the collection, exploring the genetic structure and potentially identifying the characters responsible for differentiation of the accessions. Wide phenotypic variation was observed among 51 accessions screened for 26 morphological and phenological traits. UPGMA cluster analysis and principal component analysis based on phenotypic data enabled the studied accessions to be divided into four clusters. The most important character by which the largest two clusters were differentiated was anthocyanin coloration. The clustering pattern of the AFLP dendrogram where two major clusters were identified was similar but not identical to the pattern of the phenotypic dendrogram. The Mantel test showed a high correlation between the phenotypic and the molecular data obtained (r = 0.67). In spite of the weak genetic differentiation between the accessions, STRUCTURE analysis based on AFLP data provided a clear indication for the existence of sub-groups within the two clusters. Thirty-four accessions were screened for resistance to 12 races of B. lactucae. The results show that the accessions very frequently express various reaction patterns of race specificity. Expression of race-specificity was not uniform across the set of accessions and at least 11 different reaction patterns were recorded, indicating that different racespecific resistance factors (R-factors) or genes (Dm genes) could be expected. This conclusion is supported by the results obtained in morphological and molecular studies, showing that lettuce landrace ‘Ljubljanska ledenka’ is not genetically uniform and is represented by a variety of genotypes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

GENETIC RESOURCES AND CROP EVOLUTION

ISSN

0925-9864

e-ISSN

—

Svazek periodika

68

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

19

Strana od-do

185-203

Kód UT WoS článku

000549678900001

EID výsledku v databázi Scopus

2-s2.0-85088090840