Copper(II) Complexes Containing Natural Flavonoid Pomiferin Show Considerable in Vitro Cytotoxicity and Anti-Inflammatory Effects
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F21%3A73609060" target="_blank" >RIV/61989592:15310/21:73609060 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61989592:15640/21:73609060
Výsledek na webu
<a href="https://www.mdpi.com/1422-0067/22/14/7626" target="_blank" >https://www.mdpi.com/1422-0067/22/14/7626</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms22147626" target="_blank" >10.3390/ijms22147626</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Copper(II) Complexes Containing Natural Flavonoid Pomiferin Show Considerable in Vitro Cytotoxicity and Anti-Inflammatory Effects
Popis výsledku v původním jazyce
A series of new heteroleptic copper(II) complexes of the composition [Cu(L)(bpy)]NO3 center dot 2MeOH (1), [Cu(L)(dimebpy)]NO3 center dot 2H(2)O (2), [Cu(L)(phen)]NO3 center dot 2MeOH (3), [Cu(L)(bphen)]NO3 center dot MeOH (4), [Cu(L)(dppz)]NO3 center dot MeOH (5) was prepared, where HL = 3-(3,4-dihydroxyphenyl)-5-hydroxy-8,8-dimethyl-6-(3-methylbut-2-ene-1-yl)-4H,8H-benzo[1,2-b:3,4-b ']dipyran-4-one, (pomiferin) and bpy = 2,2 '-bipyridine, dimebpy = 4,4 '-dimethyl-2,2 '-bipyridine, phen = 1,10-phenanthroline, bphen = 4,7-diphenyl-1,10-phenanthroline, and dppz = dipyrido[3,2-a:2 ',3 '-c]phenazine. The complexes were characterized using elemental analysis, infrared and UV/Vis spectroscopies, mass spectrometry, thermal analysis and conductivity measurements. The in vitro cytotoxicity, screened against eight human cancer cell lines (breast adenocarcinoma (MCF-7), osteosarcoma (HOS), lung adenocarcinoma (A549), prostate adenocarcinoma (PC-3), ovarian carcinoma (A2780), cisplatin-resistant ovarian carcinoma (A2780R), colorectal adenocarcinoma (Caco-2) and monocytic leukemia (THP-1), revealed the complexes as effective antiproliferative agents, with the IC50 values of 2.2-13.0 mu M for the best performing complexes 3 and 5. All the complexes 1-5 showed the best activity against the A2780R cells (IC50 = 2.2-6.6 mu M), and moreover, the complexes demonstrated relatively low toxicity on healthy human hepatocytes, with IC50 > 100 mu M. The complexes were evaluated by the Annexin V/propidium iodide apoptosis assay, induction of cell cycle modifications in A2780 cells, production of reactive oxygen species (ROS), perturbation of mitochondrial membrane potential, inhibition of apoptosis and inflammation-related signaling pathways (NF-kappa B/AP-1 activity, NF-kappa B translocation, TNF-alpha secretion), and tested for nuclease mimicking activity. The obtained results revealed the corresponding complexes to be effective antiproliferative and anti-inflammatory agents.
Název v anglickém jazyce
Copper(II) Complexes Containing Natural Flavonoid Pomiferin Show Considerable in Vitro Cytotoxicity and Anti-Inflammatory Effects
Popis výsledku anglicky
A series of new heteroleptic copper(II) complexes of the composition [Cu(L)(bpy)]NO3 center dot 2MeOH (1), [Cu(L)(dimebpy)]NO3 center dot 2H(2)O (2), [Cu(L)(phen)]NO3 center dot 2MeOH (3), [Cu(L)(bphen)]NO3 center dot MeOH (4), [Cu(L)(dppz)]NO3 center dot MeOH (5) was prepared, where HL = 3-(3,4-dihydroxyphenyl)-5-hydroxy-8,8-dimethyl-6-(3-methylbut-2-ene-1-yl)-4H,8H-benzo[1,2-b:3,4-b ']dipyran-4-one, (pomiferin) and bpy = 2,2 '-bipyridine, dimebpy = 4,4 '-dimethyl-2,2 '-bipyridine, phen = 1,10-phenanthroline, bphen = 4,7-diphenyl-1,10-phenanthroline, and dppz = dipyrido[3,2-a:2 ',3 '-c]phenazine. The complexes were characterized using elemental analysis, infrared and UV/Vis spectroscopies, mass spectrometry, thermal analysis and conductivity measurements. The in vitro cytotoxicity, screened against eight human cancer cell lines (breast adenocarcinoma (MCF-7), osteosarcoma (HOS), lung adenocarcinoma (A549), prostate adenocarcinoma (PC-3), ovarian carcinoma (A2780), cisplatin-resistant ovarian carcinoma (A2780R), colorectal adenocarcinoma (Caco-2) and monocytic leukemia (THP-1), revealed the complexes as effective antiproliferative agents, with the IC50 values of 2.2-13.0 mu M for the best performing complexes 3 and 5. All the complexes 1-5 showed the best activity against the A2780R cells (IC50 = 2.2-6.6 mu M), and moreover, the complexes demonstrated relatively low toxicity on healthy human hepatocytes, with IC50 > 100 mu M. The complexes were evaluated by the Annexin V/propidium iodide apoptosis assay, induction of cell cycle modifications in A2780 cells, production of reactive oxygen species (ROS), perturbation of mitochondrial membrane potential, inhibition of apoptosis and inflammation-related signaling pathways (NF-kappa B/AP-1 activity, NF-kappa B translocation, TNF-alpha secretion), and tested for nuclease mimicking activity. The obtained results revealed the corresponding complexes to be effective antiproliferative and anti-inflammatory agents.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10402 - Inorganic and nuclear chemistry
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1422-0067
e-ISSN
—
Svazek periodika
22
Číslo periodika v rámci svazku
14
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
29
Strana od-do
—
Kód UT WoS článku
000676291100001
EID výsledku v databázi Scopus
2-s2.0-85110138617