Complete genome sequence of a novel cytorhabdovirus infecting elderberry (Sambucus nigra L.) in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73612946" target="_blank" >RIV/61989592:15310/22:73612946 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007/s00705-022-05444-4.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007/s00705-022-05444-4.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00705-022-05444-4" target="_blank" >10.1007/s00705-022-05444-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Complete genome sequence of a novel cytorhabdovirus infecting elderberry (Sambucus nigra L.) in the Czech Republic

Popis výsledku v původním jazyce

The genus Cytorhabdovirus includes plant viruses with an unsegmented, single-stranded, negative-sense RNA genome that infect various plant hosts. In this work, we report the detection of a new cytorhabdovirus infecting elderberry (Sambucus nigra L.). Total RNA was purified from infected leaves and, after ribodepletion, sequenced using an Illumina system. The RNA genome of viral isolate B15 is 12,622 nucleotides (nt) long, and that of isolate B42 is 12,621 nt long. A nearly complete sequence (12,592 nt) was also obtained for a third isolate (B160). The RNA genomes of all three isolates showed an organisation typical of cytorhabdoviruses, harbouring all six of the expected genes (3 &apos; N-P-P3-M-G-L 5 &apos;), separated by intergenic regions. These isolates were closely related to each other (99.5-99.6% nt sequence identity) and showed the highest overall similarity to trichosanthes associated rhabdovirus 1 (63.5% identity) and Wuhan insect virus 5 (58% identity), and similar results were obtained when comparing individual coding sequences or proteins. Phylogenetic analysis confirmed that this elderberry virus, for which we propose the name &quot;sambucus virus 1&quot; belongs to the genus Cytorhabdovirus and fulfils the criteria to represent a novel species.

Název v anglickém jazyce

Complete genome sequence of a novel cytorhabdovirus infecting elderberry (Sambucus nigra L.) in the Czech Republic

Popis výsledku anglicky

The genus Cytorhabdovirus includes plant viruses with an unsegmented, single-stranded, negative-sense RNA genome that infect various plant hosts. In this work, we report the detection of a new cytorhabdovirus infecting elderberry (Sambucus nigra L.). Total RNA was purified from infected leaves and, after ribodepletion, sequenced using an Illumina system. The RNA genome of viral isolate B15 is 12,622 nucleotides (nt) long, and that of isolate B42 is 12,621 nt long. A nearly complete sequence (12,592 nt) was also obtained for a third isolate (B160). The RNA genomes of all three isolates showed an organisation typical of cytorhabdoviruses, harbouring all six of the expected genes (3 &apos; N-P-P3-M-G-L 5 &apos;), separated by intergenic regions. These isolates were closely related to each other (99.5-99.6% nt sequence identity) and showed the highest overall similarity to trichosanthes associated rhabdovirus 1 (63.5% identity) and Wuhan insect virus 5 (58% identity), and similar results were obtained when comparing individual coding sequences or proteins. Phylogenetic analysis confirmed that this elderberry virus, for which we propose the name &quot;sambucus virus 1&quot; belongs to the genus Cytorhabdovirus and fulfils the criteria to represent a novel species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/LD15048" target="_blank" >LD15048: Využití next generation sequencing ve studiu viromu ovocných dřevin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ARCHIVES OF VIROLOGY

ISSN

0304-8608

e-ISSN

1432-8798

Svazek periodika

167

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

4

Strana od-do

1589-1592

Kód UT WoS článku

000793012600001

EID výsledku v databázi Scopus

2-s2.0-85130038987