Scanning electrochemical microscopy-somatic cell count as a method for diagnosis of bovine mastitis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73614818" target="_blank" >RIV/61989592:15310/22:73614818 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2079-7737/11/4/549/htm" target="_blank" >https://www.mdpi.com/2079-7737/11/4/549/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/biology11040549" target="_blank" >10.3390/biology11040549</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Scanning electrochemical microscopy-somatic cell count as a method for diagnosis of bovine mastitis

Popis výsledku v původním jazyce

The method to diagnose mastitis is generally the somatic cell count (SCC) by flow cytometry measurement. When the number of somatic cells in raw milk is 2.0 × 105 cells/mL or more, the condition is referred to as mastitis. In the current study, we created a milk cell chip that serves as an electrochemical method that can be easily produced and used utilizing scanning electrochemical microscopy (SECM). The microelectrode present in the cell chip scans, and the difference between the oxygen concentration near the milk cell chip and in bulk is measured as the oxygen (O2) reduction current. We estimated the relationship between respiratory activity and the number of somatic cells in raw milk as a calibration curve, using scanning electrochemical microscopy-somatic cell count (SECM-SCC). As a result, a clear correlation was shown in the range of 104 cells/mL to 106 cells/mL. The respiration rate (F) was estimated to be about 10–16 mol/s per somatic cell. We also followed the increase in oxygen consumption during the respiratory burst using differentiation inducer phorbol 12-myristate 13-acetate (PMA) as an early stage of mastitis, accompanied with an increase in immune cells, which showed similar results. In addition, we were able to discriminate between cattle with mastitis and without mastitis.

Název v anglickém jazyce

Scanning electrochemical microscopy-somatic cell count as a method for diagnosis of bovine mastitis

Popis výsledku anglicky

The method to diagnose mastitis is generally the somatic cell count (SCC) by flow cytometry measurement. When the number of somatic cells in raw milk is 2.0 × 105 cells/mL or more, the condition is referred to as mastitis. In the current study, we created a milk cell chip that serves as an electrochemical method that can be easily produced and used utilizing scanning electrochemical microscopy (SECM). The microelectrode present in the cell chip scans, and the difference between the oxygen concentration near the milk cell chip and in bulk is measured as the oxygen (O2) reduction current. We estimated the relationship between respiratory activity and the number of somatic cells in raw milk as a calibration curve, using scanning electrochemical microscopy-somatic cell count (SECM-SCC). As a result, a clear correlation was shown in the range of 104 cells/mL to 106 cells/mL. The respiration rate (F) was estimated to be about 10–16 mol/s per somatic cell. We also followed the increase in oxygen consumption during the respiratory burst using differentiation inducer phorbol 12-myristate 13-acetate (PMA) as an early stage of mastitis, accompanied with an increase in immune cells, which showed similar results. In addition, we were able to discriminate between cattle with mastitis and without mastitis.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biology

ISSN

2079-7737

e-ISSN

2079-7737

Svazek periodika

11

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

"549-1"-"549-15"

Kód UT WoS článku

000786218800001

EID výsledku v databázi Scopus

2-s2.0-85128421986