Cooling storage of ram sperm in presence of antioxidant glutathione.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73616581" target="_blank" >RIV/61989592:15310/22:73616581 - isvavai.cz</a>

Výsledek na webu

<a href="https://cjas.agriculturejournals.cz/pdfs/cjs/2022/09/02.pdf" target="_blank" >https://cjas.agriculturejournals.cz/pdfs/cjs/2022/09/02.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/113/2022-CJAS" target="_blank" >10.17221/113/2022-CJAS</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cooling storage of ram sperm in presence of antioxidant glutathione.

Popis výsledku v původním jazyce

In the process of cryopreservation, the semen of animals can be threatened by many factors, such as oxidative damage, which affect the motility and plasma membrane function of the sperm. As an endogenous antioxidant in animals, glutathione (GSH) can significantly improve the quality of thawed sperm when added to the diluent of the semen. This study was aimed at examining the effect of GSH (given at 0.5 mM, 1.5 mM and 5 mM) on the motility and viability of ram sperm following storage under cooling conditions for several days. GSH reduced the negative effect of the cooling conditions on the total and progressive motility (CASA), stability of the membranes (annexin V/PI) and incidence of the apoptosis/necrosis (Yo-Pro-1/PI) in the sperm maintained at 5 °C for 24 h and 48 hours. These results indicate that the addition of GSH to the sperm extender can maintain the quality of the ram sperm stored at cooling conditions for at least two days.

Název v anglickém jazyce

Cooling storage of ram sperm in presence of antioxidant glutathione.

Popis výsledku anglicky

In the process of cryopreservation, the semen of animals can be threatened by many factors, such as oxidative damage, which affect the motility and plasma membrane function of the sperm. As an endogenous antioxidant in animals, glutathione (GSH) can significantly improve the quality of thawed sperm when added to the diluent of the semen. This study was aimed at examining the effect of GSH (given at 0.5 mM, 1.5 mM and 5 mM) on the motility and viability of ram sperm following storage under cooling conditions for several days. GSH reduced the negative effect of the cooling conditions on the total and progressive motility (CASA), stability of the membranes (annexin V/PI) and incidence of the apoptosis/necrosis (Yo-Pro-1/PI) in the sperm maintained at 5 °C for 24 h and 48 hours. These results indicate that the addition of GSH to the sperm extender can maintain the quality of the ram sperm stored at cooling conditions for at least two days.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CZECH JOURNAL OF ANIMAL SCIENCE

ISSN

1212-1819

e-ISSN

1805-9309

Svazek periodika

67

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

"356 "- 364

Kód UT WoS článku

000868999900002

EID výsledku v databázi Scopus

2-s2.0-85142800096