Separation of tamsulosin enantiomers by capillary electrophoresis with tandem mass spectrometry and online stacking preconcentration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F23%3A73621656" target="_blank" >RIV/61989592:15310/23:73621656 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2023/AY/D3AY00684K" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2023/AY/D3AY00684K</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d3ay00684k" target="_blank" >10.1039/d3ay00684k</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Separation of tamsulosin enantiomers by capillary electrophoresis with tandem mass spectrometry and online stacking preconcentration

Popis výsledku v původním jazyce

The aim of our work was to develop a new method for the analysis of tamsulosin enantiomers by capillary electrophoresis connected with tandem mass spectrometry. The pharmacologically active (R)-enantiomer of tamsulosin, is used to treat benign prostatic hyperplasia and chronic prostatitis. Under the optimal conditions, background electrolyte consisting of 200 mM acetic acid titrated with NH4OH to pH 4.0 containing 4.0 mg mL(-1) sulfated &amp; beta;-cyclodextrin, an injection time of 40 s at 50 mbar, a voltage of 20 kV and an optimized MS set-up (as e.g., sheath liquid containing 75 : 24.9 : 0.1 MeOH, H2O, and formic acid, v/v), a limit of detection of 1.6 nmol L-1 was achieved. The method was validated in terms of linearity, detection and quantification limits, precision, recovery, and selectivity. The results showed that the method can be used for the analysis of tamsulosin enantiomers in environmental samples, but generally, it can be applied to many different analytical tasks.

Název v anglickém jazyce

Separation of tamsulosin enantiomers by capillary electrophoresis with tandem mass spectrometry and online stacking preconcentration

Popis výsledku anglicky

The aim of our work was to develop a new method for the analysis of tamsulosin enantiomers by capillary electrophoresis connected with tandem mass spectrometry. The pharmacologically active (R)-enantiomer of tamsulosin, is used to treat benign prostatic hyperplasia and chronic prostatitis. Under the optimal conditions, background electrolyte consisting of 200 mM acetic acid titrated with NH4OH to pH 4.0 containing 4.0 mg mL(-1) sulfated &amp; beta;-cyclodextrin, an injection time of 40 s at 50 mbar, a voltage of 20 kV and an optimized MS set-up (as e.g., sheath liquid containing 75 : 24.9 : 0.1 MeOH, H2O, and formic acid, v/v), a limit of detection of 1.6 nmol L-1 was achieved. The method was validated in terms of linearity, detection and quantification limits, precision, recovery, and selectivity. The results showed that the method can be used for the analysis of tamsulosin enantiomers in environmental samples, but generally, it can be applied to many different analytical tasks.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA19-23033S" target="_blank" >GA19-23033S: Zlepšení citlivosti detekce v chirálních separacích pomocí kapilární elektroforézy ve spojení s ESI-MS nebo ICP-MS</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

1759-9679

Svazek periodika

15

Číslo periodika v rámci svazku

29

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

3543-3548

Kód UT WoS článku

001024791600001

EID výsledku v databázi Scopus

2-s2.0-85166347022