Amino acid conjugation of oxIAA is a secondary metabolic regulation involved in auxin homeostasis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F23%3A73621825" target="_blank" >RIV/61989592:15310/23:73621825 - isvavai.cz</a>

Výsledek na webu

<a href="https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.18887" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/epdf/10.1111/nph.18887</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.18887" target="_blank" >10.1111/nph.18887</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Amino acid conjugation of oxIAA is a secondary metabolic regulation involved in auxin homeostasis

Popis výsledku v původním jazyce

Dynamic regulation of the concentration of the natural auxin indole-3-acetic acid (IAA) is essential to coordinate most physiological and developmental processes and responses to environmental changes (reviewed in Friml, 2022). Auxin inactivation plays a crucial role in auxin homeostasis and metabolism. The primary enzymes involved in auxin metabolism have been known for some time. Members of the acyl acid amide synthetases belonging to the GRETCHEN HAGEN 3 (GH3), and the amidohydrolase IAA-LEUCINE RESISTANT 1 (ILR1) and ILR1-like (ILL) families catalyze the conjugation of IAA to amino acids and hydrolysis of the IAA-amino acid conjugates, respectively (LeClere et al., 2002; Staswick et al., 2005). The DIOXIGENASE FOR AUXIN OXIDATION (DAO) enzymes were shown to catalyze the oxidation of IAA to form oxIAA (Porco et al., 2016; Zhang et al., 2016). Albeit these IAA-inactivating enzymes appeared to participate in different catabolic routes, very recently, it was reported that GH3, ILR1, and DAO are part of a single linear pathway rather than two distinct pathways (Hayashi et al., 2021). According to this model, IAA is mainly inactivated by GH3 enzymes, DAO functions as an oxidase of IAA-amino acid conjugates to produce oxIAA-amino acid conjugates downstream of GH3, and oxIAA is produced from oxIAA-amino acid hydrolysis by ILR1. Therefore, DAO and ILR1 enzymes appeared to play a role in this pathway that differs from that assigned initially. Although GH3 enzymes are known to possess catalytic promiscuity accepting various substrates and amino acids, a possible additional role of GH3s in auxin inactivation was not investigated (Staswick et al., 2005; Westfall et al., 2012). Besides, while the new model proposed by Hayashi et al. (2021) was described to occur in angiosperms, whether it operates in nonflowering species remains unknown (Ross &amp; Gélinas-Marion, 2021). GRETCHEN HAGEN 3 proteins are highly conserved all over the plant kingdom, whereas DAO and DAO-like enzymes have specifically evolved with angiosperms (Okrent &amp; Wildermurth, 2011; Brunoni et al., 2020; Kaneko et al., 2020; Takehara et al., 2020). Here, we report the evidence of oxIAA-amino acid conjugation being catalyzed by the group of IAA-conjugating enzymes belonging to Group II of GH3s. Our work suggests that the contribution of this pathway to auxin homeostasis is species-dependent.

Název v anglickém jazyce

Amino acid conjugation of oxIAA is a secondary metabolic regulation involved in auxin homeostasis

Popis výsledku anglicky

Dynamic regulation of the concentration of the natural auxin indole-3-acetic acid (IAA) is essential to coordinate most physiological and developmental processes and responses to environmental changes (reviewed in Friml, 2022). Auxin inactivation plays a crucial role in auxin homeostasis and metabolism. The primary enzymes involved in auxin metabolism have been known for some time. Members of the acyl acid amide synthetases belonging to the GRETCHEN HAGEN 3 (GH3), and the amidohydrolase IAA-LEUCINE RESISTANT 1 (ILR1) and ILR1-like (ILL) families catalyze the conjugation of IAA to amino acids and hydrolysis of the IAA-amino acid conjugates, respectively (LeClere et al., 2002; Staswick et al., 2005). The DIOXIGENASE FOR AUXIN OXIDATION (DAO) enzymes were shown to catalyze the oxidation of IAA to form oxIAA (Porco et al., 2016; Zhang et al., 2016). Albeit these IAA-inactivating enzymes appeared to participate in different catabolic routes, very recently, it was reported that GH3, ILR1, and DAO are part of a single linear pathway rather than two distinct pathways (Hayashi et al., 2021). According to this model, IAA is mainly inactivated by GH3 enzymes, DAO functions as an oxidase of IAA-amino acid conjugates to produce oxIAA-amino acid conjugates downstream of GH3, and oxIAA is produced from oxIAA-amino acid hydrolysis by ILR1. Therefore, DAO and ILR1 enzymes appeared to play a role in this pathway that differs from that assigned initially. Although GH3 enzymes are known to possess catalytic promiscuity accepting various substrates and amino acids, a possible additional role of GH3s in auxin inactivation was not investigated (Staswick et al., 2005; Westfall et al., 2012). Besides, while the new model proposed by Hayashi et al. (2021) was described to occur in angiosperms, whether it operates in nonflowering species remains unknown (Ross &amp; Gélinas-Marion, 2021). GRETCHEN HAGEN 3 proteins are highly conserved all over the plant kingdom, whereas DAO and DAO-like enzymes have specifically evolved with angiosperms (Okrent &amp; Wildermurth, 2011; Brunoni et al., 2020; Kaneko et al., 2020; Takehara et al., 2020). Here, we report the evidence of oxIAA-amino acid conjugation being catalyzed by the group of IAA-conjugating enzymes belonging to Group II of GH3s. Our work suggests that the contribution of this pathway to auxin homeostasis is species-dependent.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NEW PHYTOLOGIST

ISSN

0028-646X

e-ISSN

1469-8137

Svazek periodika

238

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

2264-2270

Kód UT WoS článku

000962218400001

EID výsledku v databázi Scopus

2-s2.0-85151445359