The morphological and functional ultrastructure of cells in preimplantation embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F23%3A73622663" target="_blank" >RIV/61989592:15310/23:73622663 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61988987:17110/23:A2402N9Q

Výsledek na webu

<a href="http://www.elis.sk/download_file.php?product_id=8083&session_id=ji4p4pt495php9k5oeqb5uqtj0" target="_blank" >http://www.elis.sk/download_file.php?product_id=8083&session_id=ji4p4pt495php9k5oeqb5uqtj0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/gpb_2023011" target="_blank" >10.4149/gpb_2023011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The morphological and functional ultrastructure of cells in preimplantation embryos

Popis výsledku v původním jazyce

Cells of pre-implantation embryos are equipped with many morphological and functional systems through which they can synthesize specific proteins and effectively ensure the protection of early embryonic development. Here we present evidence for the existence of these systems in morphologically normal and abnormal bovine blastocyst stage embryos in vivo at the ultrastructural and actin cytoskeleton levels. The appearance of organelles in the trophectoderm and inner cell mass (ICM) cells, responsible for their synthetic activities and their role in the development of early bovine embryos are described. We point out the importance of endocytic processes and the participation of extracellular vesicles in the formation of intercellular contacts and homeostasis of the embryo microenvironment. Several changes in the ultrastructural morphology of embryos produced by different methods (ICSI, parthenogenetic AC/DC electrical activation, IVF with separated sperm) and freezing/thawed embryos are described. We also show alterations occurred in the organelles after viral contamination of embryos with BHV-1 and BVDV viruses. Recorded changes in organelles and appearance of cellular autophagic structures (vesicles, multivesicular bodies and autophagolysosomes) may negatively affect embryo metabolism and lead to the emergence of pathological processes in trophectoderm and ICM cells of preimplantation embryos.

Název v anglickém jazyce

The morphological and functional ultrastructure of cells in preimplantation embryos

Popis výsledku anglicky

Cells of pre-implantation embryos are equipped with many morphological and functional systems through which they can synthesize specific proteins and effectively ensure the protection of early embryonic development. Here we present evidence for the existence of these systems in morphologically normal and abnormal bovine blastocyst stage embryos in vivo at the ultrastructural and actin cytoskeleton levels. The appearance of organelles in the trophectoderm and inner cell mass (ICM) cells, responsible for their synthetic activities and their role in the development of early bovine embryos are described. We point out the importance of endocytic processes and the participation of extracellular vesicles in the formation of intercellular contacts and homeostasis of the embryo microenvironment. Several changes in the ultrastructural morphology of embryos produced by different methods (ICSI, parthenogenetic AC/DC electrical activation, IVF with separated sperm) and freezing/thawed embryos are described. We also show alterations occurred in the organelles after viral contamination of embryos with BHV-1 and BVDV viruses. Recorded changes in organelles and appearance of cellular autophagic structures (vesicles, multivesicular bodies and autophagolysosomes) may negatively affect embryo metabolism and lead to the emergence of pathological processes in trophectoderm and ICM cells of preimplantation embryos.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10604 - Reproductive biology (medical aspects to be 3)

Projekt

<a href="/cs/project/QK22010270" target="_blank" >QK22010270: Optimalizace řízení individuální reprodukční výkonnosti dojeného skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

GENERAL PHYSIOLOGY AND BIOPHYSICS

ISSN

0231-5882

e-ISSN

1338-4325

Svazek periodika

42

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

15

Strana od-do

307-321

Kód UT WoS článku

001039331300001

EID výsledku v databázi Scopus

2-s2.0-85164759234