Assessing key parameters in simultaneous simulation of rapid kinetics of chlorophyll a fluorescence and trans-thylakoid electric potential difference

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F24%3A73625793" target="_blank" >RIV/61989592:15310/24:73625793 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.14517" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.14517</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.14517" target="_blank" >10.1111/ppl.14517</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessing key parameters in simultaneous simulation of rapid kinetics of chlorophyll a fluorescence and trans-thylakoid electric potential difference

Popis výsledku v původním jazyce

Our study attempts to address the following questions: among numerous photosynthetic modules, which parameters notably influence the rapid chlorophyll fluorescence (ChlF) rise, the so-called O-J-I-P transient, in conjunction with the P515 signal, as these two records are easily obtained and widely used in photosynthesis research, and how are these parameters ranked in terms of their importance? These questions might be difficult to answer solely through experimental assays. Therefore, we employed an established photosynthesis model. Firstly, we utilized the model to simulate the measured rapid ChlF rise and P515 kinetics simultaneously. Secondly, we employed the sensitivity analysis (SA) tool by randomly altering model parameters to observe their effects on model output variables. Thirdly, we systematically identified significant parameters for both or one of the kinetics across various scenarios. A novel aspect of our study is the application of the Morris method, a global SA tool, to simultaneously assess the significance of model parameters in shaping both or one of the kinetics. The Morris SA technique enables the quantification of how much a specific parameter affects O-J-I-P transient during particular time intervals (e.g., J, I, and P steps). This allowed us to theoretically analyze which step is more significantly influenced by the parameter. In summary, our study contributes to the field by providing a comprehensive analysis of photosynthesis kinetics and emphasizing the importance of parameter selection in modelling this process. These findings can inform future research efforts aimed at improving photosynthesis models and advancing our understanding of photosynthetic processes.

Název v anglickém jazyce

Assessing key parameters in simultaneous simulation of rapid kinetics of chlorophyll a fluorescence and trans-thylakoid electric potential difference

Popis výsledku anglicky

Our study attempts to address the following questions: among numerous photosynthetic modules, which parameters notably influence the rapid chlorophyll fluorescence (ChlF) rise, the so-called O-J-I-P transient, in conjunction with the P515 signal, as these two records are easily obtained and widely used in photosynthesis research, and how are these parameters ranked in terms of their importance? These questions might be difficult to answer solely through experimental assays. Therefore, we employed an established photosynthesis model. Firstly, we utilized the model to simulate the measured rapid ChlF rise and P515 kinetics simultaneously. Secondly, we employed the sensitivity analysis (SA) tool by randomly altering model parameters to observe their effects on model output variables. Thirdly, we systematically identified significant parameters for both or one of the kinetics across various scenarios. A novel aspect of our study is the application of the Morris method, a global SA tool, to simultaneously assess the significance of model parameters in shaping both or one of the kinetics. The Morris SA technique enables the quantification of how much a specific parameter affects O-J-I-P transient during particular time intervals (e.g., J, I, and P steps). This allowed us to theoretically analyze which step is more significantly influenced by the parameter. In summary, our study contributes to the field by providing a comprehensive analysis of photosynthesis kinetics and emphasizing the importance of parameter selection in modelling this process. These findings can inform future research efforts aimed at improving photosynthesis models and advancing our understanding of photosynthetic processes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PHYSIOLOGIA PLANTARUM

ISSN

0031-9317

e-ISSN

1399-3054

Svazek periodika

176

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

20

Strana od-do

"e1451-1"-"e1451-20"

Kód UT WoS článku

001313878000001

EID výsledku v databázi Scopus

2-s2.0-85204226415