Comparative evaluation of the anti-proliferative effects of alkaloid-rich extract of jujube seed and paclitaxel on MDA-MB-231 breast cancer cell line

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F24%3A73627802" target="_blank" >RIV/61989592:15310/24:73627802 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2666154324004757" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2666154324004757</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jafr.2024.101438" target="_blank" >10.1016/j.jafr.2024.101438</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative evaluation of the anti-proliferative effects of alkaloid-rich extract of jujube seed and paclitaxel on MDA-MB-231 breast cancer cell line

Popis výsledku v původním jazyce

Introduction: Cancer is the second cause of death in the world, and among all types of cancer, triple-negative breast cancer (TNBC) has one of the worst prognoses. Repeated use of chemotherapy drugs is associated with drug resistance. This study aims to investigate the effect of jujube alkaloid-rich extract on drug sensitivity and its anti-tumor effects on paclitaxel-resistant MDA-MB-231 cells in vitro. Materials and methods: The MDA-MB-231 breast cancer cell line and its paclitaxel-resistant variant were used. The XTT method was employed to measure cell viability, while Annexin-PI assays were used to detect apoptosis. Various concentrations of the jujube extract alone and in combination with paclitaxel assessed synergistic effects in 2D and 3D cell culture models. Results: The presence of alkaloids in the extract obtained from the jujube seed was confirmed by the dragendorff test and its amount was 0.395 g in 5.0 g of jujube seed powder. Paclitaxel and jujube extract exhibited dose-dependent cytotoxic effects on paclitaxel-resistant MDA-MB-231 cells. The combination of jujube extract and paclitaxel significantly decreased IC50 from 541.3 μg/mL to 124.3 μg/mL, and the IC20 decreased from 92.1 μg/mL to 23.9 μg/mL when combined with paclitaxel in 2D culture model. The combination&apos;s toxicity was reduced in the 3D culture model. Apoptosis rates were 0.75 % in the control group, 13.89 % in the alkaloid-rich extract group, 2.59 % in the paclitaxel group, and 42.90 % in the combination group. Conclusion: Combining paclitaxel with alkaloid-rich jujube seed extract enhances cytotoxicity and apoptosis in paclitaxel-resistant MDA-MB-231 breast cancer cells in both 2D and 3D culture models. This suggests that such combinations might be a viable strategy to overcome drug resistance in TNBC treatments.

Název v anglickém jazyce

Comparative evaluation of the anti-proliferative effects of alkaloid-rich extract of jujube seed and paclitaxel on MDA-MB-231 breast cancer cell line

Popis výsledku anglicky

Introduction: Cancer is the second cause of death in the world, and among all types of cancer, triple-negative breast cancer (TNBC) has one of the worst prognoses. Repeated use of chemotherapy drugs is associated with drug resistance. This study aims to investigate the effect of jujube alkaloid-rich extract on drug sensitivity and its anti-tumor effects on paclitaxel-resistant MDA-MB-231 cells in vitro. Materials and methods: The MDA-MB-231 breast cancer cell line and its paclitaxel-resistant variant were used. The XTT method was employed to measure cell viability, while Annexin-PI assays were used to detect apoptosis. Various concentrations of the jujube extract alone and in combination with paclitaxel assessed synergistic effects in 2D and 3D cell culture models. Results: The presence of alkaloids in the extract obtained from the jujube seed was confirmed by the dragendorff test and its amount was 0.395 g in 5.0 g of jujube seed powder. Paclitaxel and jujube extract exhibited dose-dependent cytotoxic effects on paclitaxel-resistant MDA-MB-231 cells. The combination of jujube extract and paclitaxel significantly decreased IC50 from 541.3 μg/mL to 124.3 μg/mL, and the IC20 decreased from 92.1 μg/mL to 23.9 μg/mL when combined with paclitaxel in 2D culture model. The combination&apos;s toxicity was reduced in the 3D culture model. Apoptosis rates were 0.75 % in the control group, 13.89 % in the alkaloid-rich extract group, 2.59 % in the paclitaxel group, and 42.90 % in the combination group. Conclusion: Combining paclitaxel with alkaloid-rich jujube seed extract enhances cytotoxicity and apoptosis in paclitaxel-resistant MDA-MB-231 breast cancer cells in both 2D and 3D culture models. This suggests that such combinations might be a viable strategy to overcome drug resistance in TNBC treatments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30107 - Medicinal chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF AGRICULTURE AND FOOD RESEARCH

ISSN

2666-1543

e-ISSN

2666-1543

Svazek periodika

18

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

"101438-1"-"101438-9"

Kód UT WoS článku

001328182900001

EID výsledku v databázi Scopus

2-s2.0-85204871169