Constitutive and regulatory responses of Arabidopsis thaliana to harmonically oscillating light

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73631354" target="_blank" >RIV/61989592:15310/25:73631354 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.70421" target="_blank" >https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.70421</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.70421" target="_blank" >10.1111/ppl.70421</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Constitutive and regulatory responses of Arabidopsis thaliana to harmonically oscillating light

Popis výsledku v původním jazyce

The rate of net CO2 uptake is proportional to dim light and saturates when the light exceeds the plant&apos;s assimilation capacity. This simple relationship between constant light and photosynthesis becomes intriguingly complex when the light oscillates. The rates of photosynthesis may differ between the descending and ascending phases of light oscillation. This hysteresis changes with the frequency and amplitude of the light and reports on the dynamics of the photosynthetic reactions and their regulation. Here, we investigated the chlorophyll fluorescence response of Arabidopsis thaliana to light oscillating with three different amplitudes: 100–200, 100–400, and 100–800 μmol photons m−2 s−1, each with periods ranging from 1 s to 8 min. The light amplitudes and periods were chosen to represent light patterns often appearing in nature. Three genotypes were compared: wild-type Col-0 and npq1 and npq4 mutants that are incapacitated in the rapidly reversible energy-dependent non-photochemical quenching (qE). The experiments identified two major dynamic patterns. One was found in oscillation periods shorter than 30 s, characterized by constitutive hysteresis and non-linearity. The other was mainly formed by regulatory hysteresis, occurring when the oscillation periods were longer than 30 s. The mathematical model simulating the chlorophyll fluorescence dynamics qualitatively reproduced the constitutive and regulatory dynamic patterns observed in the experiments. The model simulations illustrated the dynamics of plastoquinone pool reduction and variables affecting non-photochemical quenching that form the constitutive and regulatory hysteresis types. The model simulations provided mechanistic insights into molecular processes forming the plant response to oscillating light.

Název v anglickém jazyce

Constitutive and regulatory responses of Arabidopsis thaliana to harmonically oscillating light

Popis výsledku anglicky

The rate of net CO2 uptake is proportional to dim light and saturates when the light exceeds the plant&apos;s assimilation capacity. This simple relationship between constant light and photosynthesis becomes intriguingly complex when the light oscillates. The rates of photosynthesis may differ between the descending and ascending phases of light oscillation. This hysteresis changes with the frequency and amplitude of the light and reports on the dynamics of the photosynthetic reactions and their regulation. Here, we investigated the chlorophyll fluorescence response of Arabidopsis thaliana to light oscillating with three different amplitudes: 100–200, 100–400, and 100–800 μmol photons m−2 s−1, each with periods ranging from 1 s to 8 min. The light amplitudes and periods were chosen to represent light patterns often appearing in nature. Three genotypes were compared: wild-type Col-0 and npq1 and npq4 mutants that are incapacitated in the rapidly reversible energy-dependent non-photochemical quenching (qE). The experiments identified two major dynamic patterns. One was found in oscillation periods shorter than 30 s, characterized by constitutive hysteresis and non-linearity. The other was mainly formed by regulatory hysteresis, occurring when the oscillation periods were longer than 30 s. The mathematical model simulating the chlorophyll fluorescence dynamics qualitatively reproduced the constitutive and regulatory dynamic patterns observed in the experiments. The model simulations illustrated the dynamics of plastoquinone pool reduction and variables affecting non-photochemical quenching that form the constitutive and regulatory hysteresis types. The model simulations provided mechanistic insights into molecular processes forming the plant response to oscillating light.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

<a href="/cs/project/EH23_021%2F0008909" target="_blank" >EH23_021/0008909: Interdisciplinární přístupy pro vývoj a aplikace nových materiálů v průmyslové, zemědělské a medicinské praxi</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PHYSIOLOGIA PLANTARUM

ISSN

0031-9317

e-ISSN

1399-3054

Svazek periodika

177

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

15

Strana od-do

"e70421-1"-"e70421-15"

Kód UT WoS článku

001542415500001

EID výsledku v databázi Scopus

2-s2.0-105012302067