Direction of the cross affects seed siring and progeny fitness in experimental homoploid crosses between two diploid Ficaria taxa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73631925" target="_blank" >RIV/61989592:15310/25:73631925 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1433831925000563" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1433831925000563</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ppees.2025.125901" target="_blank" >10.1016/j.ppees.2025.125901</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direction of the cross affects seed siring and progeny fitness in experimental homoploid crosses between two diploid Ficaria taxa

Popis výsledku v původním jazyce

Understanding reproductive isolation mechanisms is essential for describing the origin of new species. In flowering plants, these mechanisms significantly affect hybridization rates (frequency of hybrid formation in natural populations) and crossing success (seed set and germinability in controlled crosses) and often are associated with asymmetries in hybrid fitness. Ficaria offers a valuable system for studying hybridization and reproductive isolation, as predominantly tetraploid, widespread phylogeographic taxon (lineage) verna (V) is thought to have arisen by hybridization between two parapatric diploid taxa (lineages) calthifolia (C) and fertilis (F) followed by polyploidization. To assess the extent and directionality of reproductive isolation, we conducted controlled intra-lineage and pairwise reciprocal crosses between the aforementioned diploids, evaluated reproductive assurance (autonomous apomixis, selfing) and analysed genome size, morphology and fitness of obtained F1 progeny. No evidence of autonomous apomixis or self-fertilisation was found in the parental diploid lineages or the hybrids. The reciprocal inter-lineage crosses (F–C cross: n = 76; C–F cross: n = 45) produced viable diploid F1 progeny whose genome size was intermediate between that of the parental lineages. The above implies that all F1 progeny in reciprocal crosses originated from cross-pollination. No polyploid individuals were observed among the progeny resulting from any cross treatment. Progeny from inter-lineage crosses exhibited greater morphological variation than progeny from intra-lineage crosses. However, inter-lineage hybridisation was asymmetric. When C was the seed parent, the crosses yielded a lower number of seeds with lower germination rates and reduced fertility of established hybrids, in comparison to when F was the seed parent. Yet these hybrids exhibited greater stature and larger flowers than their reciprocals. These asymmetries likely reflect postzygotic cytonuclear incompatibilities between parental lineages. Our research highlights the importance of hybridisation and asymmetric reproductive barriers in shaping the evolution of Ficaria and establishes a basis for further studies on the genomic complexities that lead to polyploidisation.

Název v anglickém jazyce

Direction of the cross affects seed siring and progeny fitness in experimental homoploid crosses between two diploid Ficaria taxa

Popis výsledku anglicky

Understanding reproductive isolation mechanisms is essential for describing the origin of new species. In flowering plants, these mechanisms significantly affect hybridization rates (frequency of hybrid formation in natural populations) and crossing success (seed set and germinability in controlled crosses) and often are associated with asymmetries in hybrid fitness. Ficaria offers a valuable system for studying hybridization and reproductive isolation, as predominantly tetraploid, widespread phylogeographic taxon (lineage) verna (V) is thought to have arisen by hybridization between two parapatric diploid taxa (lineages) calthifolia (C) and fertilis (F) followed by polyploidization. To assess the extent and directionality of reproductive isolation, we conducted controlled intra-lineage and pairwise reciprocal crosses between the aforementioned diploids, evaluated reproductive assurance (autonomous apomixis, selfing) and analysed genome size, morphology and fitness of obtained F1 progeny. No evidence of autonomous apomixis or self-fertilisation was found in the parental diploid lineages or the hybrids. The reciprocal inter-lineage crosses (F–C cross: n = 76; C–F cross: n = 45) produced viable diploid F1 progeny whose genome size was intermediate between that of the parental lineages. The above implies that all F1 progeny in reciprocal crosses originated from cross-pollination. No polyploid individuals were observed among the progeny resulting from any cross treatment. Progeny from inter-lineage crosses exhibited greater morphological variation than progeny from intra-lineage crosses. However, inter-lineage hybridisation was asymmetric. When C was the seed parent, the crosses yielded a lower number of seeds with lower germination rates and reduced fertility of established hybrids, in comparison to when F was the seed parent. Yet these hybrids exhibited greater stature and larger flowers than their reciprocals. These asymmetries likely reflect postzygotic cytonuclear incompatibilities between parental lineages. Our research highlights the importance of hybridisation and asymmetric reproductive barriers in shaping the evolution of Ficaria and establishes a basis for further studies on the genomic complexities that lead to polyploidisation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PERSPECTIVES IN PLANT ECOLOGY EVOLUTION AND SYSTEMATICS

ISSN

1433-8319

e-ISSN

—

Svazek periodika

69

Číslo periodika v rámci svazku

NOV

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

"125901-1"-"125901-12"

Kód UT WoS článku

001597233100001

EID výsledku v databázi Scopus

2-s2.0-105018212303