Application of modified ASAP-MS technique for direct analysis of plant microsamples and its potential for single pollen grain chemical characterization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73633578" target="_blank" >RIV/61989592:15310/25:73633578 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X25013037" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X25013037</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2025.113949" target="_blank" >10.1016/j.microc.2025.113949</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of modified ASAP-MS technique for direct analysis of plant microsamples and its potential for single pollen grain chemical characterization

Popis výsledku v původním jazyce

Background: Direct chemical analysis of plant material in microscale is highly requested in modern science and practice but reliable procedures covering current needs are not fully developed yet. Analytical procedures require advanced microscopic control of sample treatment, consequent transfer to analysis and highly sensitive micro-analytical techniques and methods that are not established in analytical laboratories. Obviously, development of complex procedures based on suitable combination of novel micromanipulation procedures with direct mass spectrometry is highly requested in plant metabolomics and single cell analysis. Results: Atmospheric solids analysis probe mass spectrometry (ASAP-MS) using modified glass capillaries was used as a novel tool for the direct analysis of plant microsamples. A small hole was cross-drilled into standard soda glass capillary tube, solid sample was inserted into the capillary, and it was placed into the ion source for ASAP-MS analysis. This approach allows direct, fast and sensitive sterol profiling in plant microsamples. Application of cyclic ion mobility enabled separation of selected phenolic compounds and consequently their reliable identification in pea seed coat fragments. A combination of the modified ASAP-MS technique and electronically driven micromanipulation sampling under microscopic control allowed analysis of separated pollen grains. Biomarkers of pollen species were identified and chemical differences among pinophyta and magnoliophyta were explored. Normalized intensities of identified fatty acids, fatty alcohols and their esters, sterol lipids, phytosterols and esters of fatty acids with phytosterols and terpenoids were significantly higher in magnoliophyta. Significance: Developed procedure allowed analysis of fatty compounds in single pollen grains. ASAP-MS provided new information about chemical composition of studied pollen species in context with plant phylogeny. Obtained data contribute to understanding of chemical stability and properties of seed and pollen surface structures. Combination of electronically driven sample micromanipulation with ASAP-MS proved to be suitable procedure for micro-metabolomic studies.

Název v anglickém jazyce

Application of modified ASAP-MS technique for direct analysis of plant microsamples and its potential for single pollen grain chemical characterization

Popis výsledku anglicky

Background: Direct chemical analysis of plant material in microscale is highly requested in modern science and practice but reliable procedures covering current needs are not fully developed yet. Analytical procedures require advanced microscopic control of sample treatment, consequent transfer to analysis and highly sensitive micro-analytical techniques and methods that are not established in analytical laboratories. Obviously, development of complex procedures based on suitable combination of novel micromanipulation procedures with direct mass spectrometry is highly requested in plant metabolomics and single cell analysis. Results: Atmospheric solids analysis probe mass spectrometry (ASAP-MS) using modified glass capillaries was used as a novel tool for the direct analysis of plant microsamples. A small hole was cross-drilled into standard soda glass capillary tube, solid sample was inserted into the capillary, and it was placed into the ion source for ASAP-MS analysis. This approach allows direct, fast and sensitive sterol profiling in plant microsamples. Application of cyclic ion mobility enabled separation of selected phenolic compounds and consequently their reliable identification in pea seed coat fragments. A combination of the modified ASAP-MS technique and electronically driven micromanipulation sampling under microscopic control allowed analysis of separated pollen grains. Biomarkers of pollen species were identified and chemical differences among pinophyta and magnoliophyta were explored. Normalized intensities of identified fatty acids, fatty alcohols and their esters, sterol lipids, phytosterols and esters of fatty acids with phytosterols and terpenoids were significantly higher in magnoliophyta. Significance: Developed procedure allowed analysis of fatty compounds in single pollen grains. ASAP-MS provided new information about chemical composition of studied pollen species in context with plant phylogeny. Obtained data contribute to understanding of chemical stability and properties of seed and pollen surface structures. Combination of electronically driven sample micromanipulation with ASAP-MS proved to be suitable procedure for micro-metabolomic studies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA24-10730S" target="_blank" >GA24-10730S: Hilum - Achilova pata semene? Záhada formování hila a funkce polyfenoloxidasy v semenech.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROCHEMICAL JOURNAL

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

214

Číslo periodika v rámci svazku

JUL

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

"113949-1"-"113949-11"

Kód UT WoS článku

001494743700003

EID výsledku v databázi Scopus

2-s2.0-105005073444