Relationships among morphological characters, isozymes polymorphism and DNA variability # the impact on Lactuca germplasm taxonomy.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15410%2F03%3A00001335" target="_blank" >RIV/61989592:15410/03:00001335 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Relationships among morphological characters, isozymes polymorphism and DNA variability # the impact on Lactuca germplasm taxonomy.

Popis výsledku v původním jazyce

Fifty one accessions of nineteen Lactuca species, hybrid of L. serriola x L. sativa and related species Mycelis muralis were evaluated for morphological variability, esterases (EST) polymorphism, AFLP (Amplified Fragment Length Polymorphism) and relativeDNA content. 16 Lactuca accessions were taxonomically redetermined on the basis of morphological studies combined with isozyme analysis and AFLP. Twenty-eight bands (isoforms) of EST were recorded giving the possibility to distinguish 82% of accessions.Relative DNA content established by flow-cytometry (DAPI staining) ranged from 2.02 pg in L. capensis to 17.96 pg in L. canadensis. Results on AFLP and relative DNA content measurement correspond well with recent taxonomic classification of the genus Lactuca.

Název v anglickém jazyce

Relationships among morphological characters, isozymes polymorphism and DNA variability # the impact on Lactuca germplasm taxonomy.

Popis výsledku anglicky

Fifty one accessions of nineteen Lactuca species, hybrid of L. serriola x L. sativa and related species Mycelis muralis were evaluated for morphological variability, esterases (EST) polymorphism, AFLP (Amplified Fragment Length Polymorphism) and relativeDNA content. 16 Lactuca accessions were taxonomically redetermined on the basis of morphological studies combined with isozyme analysis and AFLP. Twenty-eight bands (isoforms) of EST were recorded giving the possibility to distinguish 82% of accessions.Relative DNA content established by flow-cytometry (DAPI staining) ranged from 2.02 pg in L. capensis to 17.96 pg in L. canadensis. Results on AFLP and relative DNA content measurement correspond well with recent taxonomic classification of the genus Lactuca.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Czech J. Genet. Plant Breed.

ISSN

0862-8629

e-ISSN

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Svazek periodika

39

Číslo periodika v rámci svazku

N

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

59-67

Kód UT WoS článku

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EID výsledku v databázi Scopus

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