Isolation, identification and biological activity of new endogenous cytokinin class

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15410%2F03%3A00001337" target="_blank" >RIV/61989592:15410/03:00001337 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/03:00001337

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Isolation, identification and biological activity of new endogenous cytokinin class

Popis výsledku v původním jazyce

Our research of naturally occuring cytokinins in plants led to the discovery of four new plant hormone substances: 6-(2-methoxybenzylamino)purine and 6-(3-methoxybenzylamino)purine and their 9-b-D-ribofuranosyl derivates. These substances were identifiedin Arabidopsis thaliana plants and Populus x canadensis leaves by capillary-liquid chromatography/ frit-fast atom bombardment - mass spectrometry after precolumn derivatization. Endogenous levels of the methoxytopolins were studied using enzyme-linked immunosorbent assays of HPLC-fractionated extracts. Their high cytokinin activity was demonstrated in three bioassays testing their ability to stimulate tobacco callus growth, to delay chlorophyll degradation in excised wheat leaves, and to induce betacyanin synthesis in Amaranthus caudatus var. atropurpurea cotyledons.The results showed particularly their great potential as anti-senescence factors. Moreover, the ability of new derivatives (in micromolar concentrations) to inhibit growth

Název v anglickém jazyce

Isolation, identification and biological activity of new endogenous cytokinin class

Popis výsledku anglicky

Our research of naturally occuring cytokinins in plants led to the discovery of four new plant hormone substances: 6-(2-methoxybenzylamino)purine and 6-(3-methoxybenzylamino)purine and their 9-b-D-ribofuranosyl derivates. These substances were identifiedin Arabidopsis thaliana plants and Populus x canadensis leaves by capillary-liquid chromatography/ frit-fast atom bombardment - mass spectrometry after precolumn derivatization. Endogenous levels of the methoxytopolins were studied using enzyme-linked immunosorbent assays of HPLC-fractionated extracts. Their high cytokinin activity was demonstrated in three bioassays testing their ability to stimulate tobacco callus growth, to delay chlorophyll degradation in excised wheat leaves, and to induce betacyanin synthesis in Amaranthus caudatus var. atropurpurea cotyledons.The results showed particularly their great potential as anti-senescence factors. Moreover, the ability of new derivatives (in micromolar concentrations) to inhibit growth

Druh

D - Stať ve sborníku

CEP obor

EF - Botanika

OECD FORD obor

—

Projekt

<a href="/cs/project/GA522%2F01%2F0275" target="_blank" >GA522/01/0275: Studium nových cytokininových metabolitů v rostlinách: isolace, identifikace a biosyntéza</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Plant Biology 2003 - the 2003 annual meeting of the American Society of Plant Biology

ISBN

—

ISSN

—

e-ISSN

—

Počet stran výsledku

286

Strana od-do

150

Název nakladatele

American Society of Plant Physiologists

Místo vydání

Honolulu

Místo konání akce

Honolulu

Datum konání akce

—

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—