C-geranylated flavonoids from Paulownia tomentosa Steud. fruit as potential anti-inflammatory agents

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F22%3A73617078" target="_blank" >RIV/61989592:15640/22:73617078 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14160/22:00129753 RIV/60460709:41210/22:92271

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0378874122005487?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0378874122005487?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jep.2022.115509" target="_blank" >10.1016/j.jep.2022.115509</a>

Jazyk výsledku

angličtina

Název v původním jazyce

C-geranylated flavonoids from Paulownia tomentosa Steud. fruit as potential anti-inflammatory agents

Popis výsledku v původním jazyce

Ethnopharmacological relevance: Paulownia tomentosa Steud., a traditional Chinese medicinal plant, was used for many centuries in Chinese herbal medicine as a component of remedies for many illnesses, including inflammatory diseases. It is a rich source of phenolic compounds, mainly geranylated flavonoids, which are currently studied for their promising biological activities. Aim of the study: The study aimed to isolate minor geranylated flavanones and flavones from P. tomentosa fruit and evaluate their cytotoxicity and possible anti-inflammatory effects in a cell-based model of inflammation. Materials and methods: Chromatographic separation of chloroform portion of the ethanolic extract of P. tomentosa fruit led to the isolation of twenty-seven flavonoids (1-27), twenty-six of them geranylated with different modifications and one non-geranylated flavanone, and two phenolic compounds. Compounds were identified using UV, IR, HRMS, NMR, and CD spectroscopy. Ten of these compounds (7-10, 12, 21, 22, 24, 25, and 27) were determined to be new flavonoid derivatives obtained from a natural source for the first time. Selected compounds were analyzed for cytotoxicity and anti-inflammatory potential to affect the activation of nuclear factor KB/activator protein 1 (NF-KB/AP-1) after lipopolysaccharide (LPS) stimulation. Results: All the test compounds (1-21 and 23-26) reduced the activation of NF-KB/AP-1 24 h after the addition of LPS. Eight compounds (5, 14-18, 21, and 26) were more active than prednisone, a widely used antiinflammatory drug. However, this effect was not seen significantly on the level of TNF-alpha and IL-1fl, which can be explained by the plurality of possible outcomes of activation of the NF-KB pathway in cells. Conclusions: Results of the presented study confirmed that constituents from traditional Chinese medicinal plant P. tomentosa Steud. have promising anti-inflammatory activities and can serve as a potential source of inspiration for new anti-inflammatory medications.

Název v anglickém jazyce

C-geranylated flavonoids from Paulownia tomentosa Steud. fruit as potential anti-inflammatory agents

Popis výsledku anglicky

Ethnopharmacological relevance: Paulownia tomentosa Steud., a traditional Chinese medicinal plant, was used for many centuries in Chinese herbal medicine as a component of remedies for many illnesses, including inflammatory diseases. It is a rich source of phenolic compounds, mainly geranylated flavonoids, which are currently studied for their promising biological activities. Aim of the study: The study aimed to isolate minor geranylated flavanones and flavones from P. tomentosa fruit and evaluate their cytotoxicity and possible anti-inflammatory effects in a cell-based model of inflammation. Materials and methods: Chromatographic separation of chloroform portion of the ethanolic extract of P. tomentosa fruit led to the isolation of twenty-seven flavonoids (1-27), twenty-six of them geranylated with different modifications and one non-geranylated flavanone, and two phenolic compounds. Compounds were identified using UV, IR, HRMS, NMR, and CD spectroscopy. Ten of these compounds (7-10, 12, 21, 22, 24, 25, and 27) were determined to be new flavonoid derivatives obtained from a natural source for the first time. Selected compounds were analyzed for cytotoxicity and anti-inflammatory potential to affect the activation of nuclear factor KB/activator protein 1 (NF-KB/AP-1) after lipopolysaccharide (LPS) stimulation. Results: All the test compounds (1-21 and 23-26) reduced the activation of NF-KB/AP-1 24 h after the addition of LPS. Eight compounds (5, 14-18, 21, and 26) were more active than prednisone, a widely used antiinflammatory drug. However, this effect was not seen significantly on the level of TNF-alpha and IL-1fl, which can be explained by the plurality of possible outcomes of activation of the NF-KB pathway in cells. Conclusions: Results of the presented study confirmed that constituents from traditional Chinese medicinal plant P. tomentosa Steud. have promising anti-inflammatory activities and can serve as a potential source of inspiration for new anti-inflammatory medications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30107 - Medicinal chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Ethnopharmacology

ISSN

0378-8741

e-ISSN

1872-7573

Svazek periodika

296

Číslo periodika v rámci svazku

October

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

14

Strana od-do

nestrankovano

Kód UT WoS článku

000830216700002

EID výsledku v databázi Scopus

2-s2.0-85133831685