The occurence of PPV in cherry trees in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A_____%2F03%3A00001652" target="_blank" >RIV/61989592:_____/03:00001652 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The occurence of PPV in cherry trees in the Czech Republic

Popis výsledku v původním jazyce

Plum pox virus (PPV) is the causal agent of Sharka disease, one of the most harmful viral diseases of stone fruits. After the first reports of PPV on sour and sweet cherry in Moldova and Italy in 1994, the virus has been recently reported in cherry in Hungary and Bulgary (1998) and in Romania (2002). Sharka is currently considered one of the most injurious virus diseases of plum, apricot and peach in the Czech Republic. In order to check the presence and distribution of PPV in cherry, from 1998 a survey in sweet and sour cherry orchards has been carried out in the Czech Republic. 46 cherry trees showing typical Sharka symptoms (light green, yellow irregular vein banding and ring-spots) have been selected in 1999. Samples were analysed by RT-PCR usingPPV 3'NCR specific primers on total nucleic acids extracted from leaves. 7 of the 42 analysed samples were positive and 2 of the respective PCR products were cloned into TA cloning kit (Invitrogen) and sequenced.

Název v anglickém jazyce

The occurence of PPV in cherry trees in the Czech Republic

Popis výsledku anglicky

Plum pox virus (PPV) is the causal agent of Sharka disease, one of the most harmful viral diseases of stone fruits. After the first reports of PPV on sour and sweet cherry in Moldova and Italy in 1994, the virus has been recently reported in cherry in Hungary and Bulgary (1998) and in Romania (2002). Sharka is currently considered one of the most injurious virus diseases of plum, apricot and peach in the Czech Republic. In order to check the presence and distribution of PPV in cherry, from 1998 a survey in sweet and sour cherry orchards has been carried out in the Czech Republic. 46 cherry trees showing typical Sharka symptoms (light green, yellow irregular vein banding and ring-spots) have been selected in 1999. Samples were analysed by RT-PCR usingPPV 3'NCR specific primers on total nucleic acids extracted from leaves. 7 of the 42 analysed samples were positive and 2 of the respective PCR products were cloned into TA cloning kit (Invitrogen) and sequenced.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/QD1407" target="_blank" >QD1407: Výběr odolných genotypů slivoní k šarce.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

19th International Symposium on Virus and Virus-like Diseases of Temporate Fruit Crops, 10th International Symposium on Small Fruit Virus Diseases.

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

75

Název nakladatele

International Society for Horticultural Science

Místo vydání

Valencia, Spain

Místo konání akce

Valencia, Spain

Datum konání akce

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Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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