CE stanovení disociačních konstant

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F04%3A00000829" target="_blank" >RIV/62156489:43210/04:00000829 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Capillary Electrophoretic Studies of Acid-Base Properties of Sanguinarine and Chelerythrine Alkaloids.

Popis výsledku v původním jazyce

Capillary zone electrophoresis with UV detection was used for determination of dissociation constants of alkaloids sanguinarine and chelerythrine. Despite the limited solubility of the uncharged forms of the alkaloids resulting in insufficient analyticalsignal at higher pH the reliable dissociation constants were obtained when acidified samples containing low amount of the alkaloid were injected into the capillary. The precipitation of the alkaloid in the capillary induced by injecting sample of low pHinto the background electrolyte of higher pH does not affect the mobility of the alkaloid if its concentration injected exceeds the solubility only to a small extent. Dissociation constants (pKR+) of sanguinarine and chelerythrine calculated to 8.3 +- 0.1 and 9.2 +- 0.1, respectively, are relevant to Good buffers of ionic strength of 30 mM.

Název v anglickém jazyce

Capillary Electrophoretic Studies of Acid-Base Properties of Sanguinarine and Chelerythrine Alkaloids.

Popis výsledku anglicky

Capillary zone electrophoresis with UV detection was used for determination of dissociation constants of alkaloids sanguinarine and chelerythrine. Despite the limited solubility of the uncharged forms of the alkaloids resulting in insufficient analyticalsignal at higher pH the reliable dissociation constants were obtained when acidified samples containing low amount of the alkaloid were injected into the capillary. The precipitation of the alkaloid in the capillary induced by injecting sample of low pHinto the background electrolyte of higher pH does not affect the mobility of the alkaloid if its concentration injected exceeds the solubility only to a small extent. Dissociation constants (pKR+) of sanguinarine and chelerythrine calculated to 8.3 +- 0.1 and 9.2 +- 0.1, respectively, are relevant to Good buffers of ionic strength of 30 mM.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1040

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

141-145

Kód UT WoS článku

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EID výsledku v databázi Scopus

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