Současné femtomolové stanovení cysteinu, redukovaného a oxidovaného glutathionu a fytochelatinů v obilkách kukuřice (Zea mays L.)za použití vysokoúčinné kapalinové chromatografie s elektrochemickou detekcí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00005198" target="_blank" >RIV/62156489:43210/05:00005198 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous femtomole determination of cysteine, reduced andoxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high performance liquid chromatography with electrochemical detection

Popis výsledku v původním jazyce

Thiol compounds such as cysteine (Cys), reduced (GSH) and oxidized (GSSG) gluathione, and phytochelatins (PCs) play an important role in heavy metal detoxification in plants. These thiols are biological active compounds whose function is elimination of oxidative stress in plant cells. The aim of our work was to optimise sensitive and rapid method of high performance liquid chromatography coupled with electrochemical detector (HPLC ED) for determination of the abovementioned thiol compounds in maize (Zeamays L.) kernels. Newapproach for evaluation of HPLC ED parameters is described. The most suitable isocratic mobile phase for the separation and detection of Cys, GSH, GSSG and PC2 consisted of methanol (MeOH) and trifluoroacetic acid (TFA). In addition, the influence of concentrations of TFA and ratio of MeOH:TFA on chromatographic separation and detection of the thiol compounds were studied. The mobile phase consisting from methanol and 0,05% (v/v) TFA in ratio 97:3 (%; v/v) was found

Název v anglickém jazyce

Simultaneous femtomole determination of cysteine, reduced andoxidized glutathione, and phytochelatin in maize (Zea mays L.) kernels using high performance liquid chromatography with electrochemical detection

Popis výsledku anglicky

Thiol compounds such as cysteine (Cys), reduced (GSH) and oxidized (GSSG) gluathione, and phytochelatins (PCs) play an important role in heavy metal detoxification in plants. These thiols are biological active compounds whose function is elimination of oxidative stress in plant cells. The aim of our work was to optimise sensitive and rapid method of high performance liquid chromatography coupled with electrochemical detector (HPLC ED) for determination of the abovementioned thiol compounds in maize (Zeamays L.) kernels. Newapproach for evaluation of HPLC ED parameters is described. The most suitable isocratic mobile phase for the separation and detection of Cys, GSH, GSSG and PC2 consisted of methanol (MeOH) and trifluoroacetic acid (TFA). In addition, the influence of concentrations of TFA and ratio of MeOH:TFA on chromatographic separation and detection of the thiol compounds were studied. The mobile phase consisting from methanol and 0,05% (v/v) TFA in ratio 97:3 (%; v/v) was found

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1084

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

134-144

Kód UT WoS článku

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EID výsledku v databázi Scopus

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