Vylepšená metoda pro řízenou evoluci enzymů

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00117762" target="_blank" >RIV/62156489:43210/07:00117762 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Improved method for directed enzyme evolution

Popis výsledku v původním jazyce

In plants, beta-glucosidases have been implicated in regulating several aspects of development, including phytohormone activation, degradation of endosperm cell wall during germination, and pathogen defense. beta-glucosidases are a widespread group of enzymes that hydrolyze a broad variety of glucosides including aryl- and alkyl-beta-D-glucosides. The maize beta-glucosidase Zm-p60.1 is important for the regulation of plant development through its role in the targeted release of free cytokinins from cytokinin-O-glucosides, their inactive storage forms. For research focused on beta-glucosidase Zm-p60.1 substrate specificity we needed a procedure proving structure stability of the enzyme during mutagenesis. We developed a directed mutagenesis assay usinga system comprising specially designed multi degenerated primers and direct screening of colonies containing positive mutations. This method performed in cycles is a suitable tool for directed evolution of enzymes concretely beta-glucosid

Název v anglickém jazyce

Improved method for directed enzyme evolution

Popis výsledku anglicky

In plants, beta-glucosidases have been implicated in regulating several aspects of development, including phytohormone activation, degradation of endosperm cell wall during germination, and pathogen defense. beta-glucosidases are a widespread group of enzymes that hydrolyze a broad variety of glucosides including aryl- and alkyl-beta-D-glucosides. The maize beta-glucosidase Zm-p60.1 is important for the regulation of plant development through its role in the targeted release of free cytokinins from cytokinin-O-glucosides, their inactive storage forms. For research focused on beta-glucosidase Zm-p60.1 substrate specificity we needed a procedure proving structure stability of the enzyme during mutagenesis. We developed a directed mutagenesis assay usinga system comprising specially designed multi degenerated primers and direct screening of colonies containing positive mutations. This method performed in cycles is a suitable tool for directed evolution of enzymes concretely beta-glucosid

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/LC06034" target="_blank" >LC06034: Regulace morfogeneze rostlinných buněk a orgánů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet'07 Agro

ISBN

978-80-7375-119-7

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

108-108

Název nakladatele

MZLU v Brno

Místo vydání

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Místo konání akce

Brno

Datum konání akce

1. 1. 2007

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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