Výzkum efektu inhibice syntézi glutathionu v časných somatických embriích smrku ovlivněných kademnatými ionty

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00133467" target="_blank" >RIV/62156489:43210/08:00133467 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Investigation of effects of glutathione synthesis inhibition on early somatic embryos treated with cadmium (II) ions

Popis výsledku v původním jazyce

Glutathione can be considered as ubiquitous molecule because of its presence in all living organisms even at units of mM. The main aim of this work was to investigate the effect of glutathione biosynthesis inhibitor buthione sulfoximine (BSO) on total content of mRNA and glutathione at early somatic embryos of Spruce treated with various doses of cadmium(II) ions. Primarily we investigated the influence of BSO on the total content of mRNA (transcriptome) at ESEs. We found that the level of total mRNA enhanced for more than 10% at BSO treated ESEs compared to control embryos. At the end of the treatment the highest BSO dose (500 uM) stimulated synthesis of mRNA four times compared to control embryos. Furthermore we aimed our attention on ESEs treated with BSO + cadmium(II) ions. The highest enhancing of transcriptome level was determined at ESEs treated with 500 uM of cadmium(II) ions. Four days after application of the highest dose of BSO + cadmium(II) ions the level of transcriptome i

Název v anglickém jazyce

Investigation of effects of glutathione synthesis inhibition on early somatic embryos treated with cadmium (II) ions

Popis výsledku anglicky

Glutathione can be considered as ubiquitous molecule because of its presence in all living organisms even at units of mM. The main aim of this work was to investigate the effect of glutathione biosynthesis inhibitor buthione sulfoximine (BSO) on total content of mRNA and glutathione at early somatic embryos of Spruce treated with various doses of cadmium(II) ions. Primarily we investigated the influence of BSO on the total content of mRNA (transcriptome) at ESEs. We found that the level of total mRNA enhanced for more than 10% at BSO treated ESEs compared to control embryos. At the end of the treatment the highest BSO dose (500 uM) stimulated synthesis of mRNA four times compared to control embryos. Furthermore we aimed our attention on ESEs treated with BSO + cadmium(II) ions. The highest enhancing of transcriptome level was determined at ESEs treated with 500 uM of cadmium(II) ions. Four days after application of the highest dose of BSO + cadmium(II) ions the level of transcriptome i

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F07%2F0692" target="_blank" >GA522/07/0692: Studium tvorby thiolových sloučenin u rostlin - uplatnění při remediačních technologiích</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

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Svazek periodika

180

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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