Studium interakci mezi matrixovými metaloproteinásami s kolagen pomocí chronopotenciometrie
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00134121" target="_blank" >RIV/62156489:43210/08:00134121 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
An Investigation of interactions of matrix metalloproteinases with collagen by chronopotentiometric stripping analysis
Popis výsledku v původním jazyce
Matrix metalloproteinases (MMPs) represent a family of structurally related zinc-dependent metallopeptidases. MMPs are able to degrade both majority and minority components of extracellular matrix include the native helix of collagen. These enzymes takepart in normal biological processes such as ontogenesis or wound healing but also in inflammatory, generative, and especially malign processes. Zinc ion plays structural and regulatory role in MMPs but it is mainly involved directly in the catalytic hydrolysis of protein substrates. The aim of this work was to propose and optimize the chronopotentiometric stripping analysis (CPSA) in connection with adsorptive transfer technique (AdTS) for MMP-9 detection. The method has been further employed to investigate MMP-9 interaction with collagen. Thanks to AdTS CPSA at hanging mercury drop electrode we were able to measure 5 ul sample volume. We optimized the following parameters: time of the accumulation of the sample on HMDE surface and the
Název v anglickém jazyce
An Investigation of interactions of matrix metalloproteinases with collagen by chronopotentiometric stripping analysis
Popis výsledku anglicky
Matrix metalloproteinases (MMPs) represent a family of structurally related zinc-dependent metallopeptidases. MMPs are able to degrade both majority and minority components of extracellular matrix include the native helix of collagen. These enzymes takepart in normal biological processes such as ontogenesis or wound healing but also in inflammatory, generative, and especially malign processes. Zinc ion plays structural and regulatory role in MMPs but it is mainly involved directly in the catalytic hydrolysis of protein substrates. The aim of this work was to propose and optimize the chronopotentiometric stripping analysis (CPSA) in connection with adsorptive transfer technique (AdTS) for MMP-9 detection. The method has been further employed to investigate MMP-9 interaction with collagen. Thanks to AdTS CPSA at hanging mercury drop electrode we were able to measure 5 ul sample volume. We optimized the following parameters: time of the accumulation of the sample on HMDE surface and the
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/IAA401990701" target="_blank" >IAA401990701: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2008
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
12th International Conference on Electroanalysis
ISBN
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ISSN
0009-2770
e-ISSN
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Počet stran výsledku
1
Strana od-do
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Název nakladatele
Czech Chemical Society
Místo vydání
Prague, Czech Republic
Místo konání akce
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Datum konání akce
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Typ akce podle státní příslušnosti
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Kód UT WoS článku
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