Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F11%3A00165366" target="_blank" >RIV/62156489:43210/11:00165366 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/10:00353420

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study

Popis výsledku v původním jazyce

Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96CG in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829TC in the coding sequence with allele C present only in Meishan. Linkage mapping placed the IRS4 gene at position 82 cM on the current USDA--USMARC linkage map of porcine chromosome X. Association analyses were performed on 555 animals of 12th--15th generation of the Meishan 9 Large White cross and showed that both SNPs were highly significantly associated with backfat depth (P = 0.0005) and that the SNP FN424076:g1829TC

Název v anglickém jazyce

Porcine insulin receptor substrate 4 (IRS4) gene: cloning, polymorphism and association study

Popis výsledku anglicky

Using PCR and inverse PCR techniques we obtained a 4,498 bp nucleotide sequence FN424076 encompassing the complete coding sequence of the porcine insulin receptor substrate 4 (IRS4) gene and its proximal promoter. The 1,269 amino acid porcine protein deduced from the nucleotide sequence shares 92% identity with the human IRS4 and possesses the same domains and the same number of tyrosine phosphorylation motifs as the human protein. We detected substitution FN424076:g.96CG in the promoter region that segregates in Meishan and a synonymous substitution FN424076:g.1829TC in the coding sequence with allele C present only in Meishan. Linkage mapping placed the IRS4 gene at position 82 cM on the current USDA--USMARC linkage map of porcine chromosome X. Association analyses were performed on 555 animals of 12th--15th generation of the Meishan 9 Large White cross and showed that both SNPs were highly significantly associated with backfat depth (P = 0.0005) and that the SNP FN424076:g1829TC

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology Reports

ISSN

0301-4851

e-ISSN

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Svazek periodika

38

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

2611-2617

Kód UT WoS článku

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EID výsledku v databázi Scopus

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