Femtogram electroanalytical detection of prostatic specific antigen by Brdicka reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00184857" target="_blank" >RIV/62156489:43210/12:00184857 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/12:PU95568 RIV/00216224:14110/12:00059530 RIV/00216208:11310/12:10126268 RIV/00216208:11130/12:8024 RIV/00064203:_____/12:8024

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Femtogram electroanalytical detection of prostatic specific antigen by Brdicka reaction

Popis výsledku v původním jazyce

Prostatic-specific antigen is considered as the best marker for prostate cancer. Due to the importance of PSA for diagnostic purposes it is not surprising that there are tested and optimized various methods for its determination. In spite of such intensive research in the field of electrochemical detection of some by-products connected with concentration of PSA, electrochemical behaviour of PSA has not been studied yet. The aim of this study was to investigate electrochemical catalytic signals of PSA using differential pulse voltammetry Brdicka reaction. The catalytic signals were studied using adsorptive transfer stripping technique as well as directly in the electrochemical cell. Nevertheless, we primarily tested detection of PSA by standard immunoanalysis and by gel and capillary chip electrophoresis to investigate behaviour of this protein in electric field. Both electrophoretic methods showed that the most intensive band of PSA was determined at 37 kDa under reducing conditions an

Název v anglickém jazyce

Femtogram electroanalytical detection of prostatic specific antigen by Brdicka reaction

Popis výsledku anglicky

Prostatic-specific antigen is considered as the best marker for prostate cancer. Due to the importance of PSA for diagnostic purposes it is not surprising that there are tested and optimized various methods for its determination. In spite of such intensive research in the field of electrochemical detection of some by-products connected with concentration of PSA, electrochemical behaviour of PSA has not been studied yet. The aim of this study was to investigate electrochemical catalytic signals of PSA using differential pulse voltammetry Brdicka reaction. The catalytic signals were studied using adsorptive transfer stripping technique as well as directly in the electrochemical cell. Nevertheless, we primarily tested detection of PSA by standard immunoanalysis and by gel and capillary chip electrophoresis to investigate behaviour of this protein in electric field. Both electrophoretic methods showed that the most intensive band of PSA was determined at 37 kDa under reducing conditions an

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

18

Strana od-do

1749-1766

Kód UT WoS článku

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EID výsledku v databázi Scopus

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