Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F12%3A00198107" target="_blank" >RIV/62156489:43210/12:00198107 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/12:PU99110

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry

Popis výsledku v původním jazyce

Phytoremediation of heavy metals includes phytoextraction and phytoaccumulation, i.e. accumulation of pollution from soils or water into the plants. Inorganic pollutants like heavy metals cannot be chemically degraded and thus it has to be accumulated inroots and leaves of the plants in non-toxic forms. In this study, the influence of cadmium on transgenic Nicotiana tabacum plants carrying yeast metallothionein was investigated. Tobacco plants were exposed to 0, 50, 100 and 250 uM CdCl2 for 96 hours. Growth parameters as well as changes in content of cysteine, oxidized/reduced glutathione, phytochelatins (PC2, PC3 and PC4) were monitored using high performance liquid chromatography with electrochemical detection. Moreover, we exploited differential pulse voltammetry Brdicka reaction for determination of metallothionein and verified the results obtained by dot blot assay. In addition, all results were compared with results obtained using non-transgenic tobacco plants. Our results indic

Název v anglickém jazyce

Accumulation of cadmium by transgenic tobacco plants (Nicotiana tabacum L.) carrying yeast metallothionein gene revealed by electrochemistry

Popis výsledku anglicky

Phytoremediation of heavy metals includes phytoextraction and phytoaccumulation, i.e. accumulation of pollution from soils or water into the plants. Inorganic pollutants like heavy metals cannot be chemically degraded and thus it has to be accumulated inroots and leaves of the plants in non-toxic forms. In this study, the influence of cadmium on transgenic Nicotiana tabacum plants carrying yeast metallothionein was investigated. Tobacco plants were exposed to 0, 50, 100 and 250 uM CdCl2 for 96 hours. Growth parameters as well as changes in content of cysteine, oxidized/reduced glutathione, phytochelatins (PC2, PC3 and PC4) were monitored using high performance liquid chromatography with electrochemical detection. Moreover, we exploited differential pulse voltammetry Brdicka reaction for determination of metallothionein and verified the results obtained by dot blot assay. In addition, all results were compared with results obtained using non-transgenic tobacco plants. Our results indic

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

22

Strana od-do

886-907

Kód UT WoS článku

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EID výsledku v databázi Scopus

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