Using of paramagnetic microparticles and quantum dots for isolation and electrochemical detection of influenza viruses' specific nucleic acids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00193692" target="_blank" >RIV/62156489:43210/13:00193692 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/13:PU103043

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Using of paramagnetic microparticles and quantum dots for isolation and electrochemical detection of influenza viruses' specific nucleic acids

Popis výsledku v původním jazyce

The aim of this study was to suggest isolation protocol based on paramagnetic microparticles and multicolour electrochemical detection of three various influenza oligonucleotides (H1N1 influenza subtype, H3N2 influenza subtype and H5N1 influenza subtype)modified with nanoparticles (NPs) called quantum dots (QDs), which are made from zinc, cadmium or lead. Particularly, we described hybridization assay based on paramagnetic microparticles (MPs) and coupled with detection of three different influenza derived oligonucleotides (ODNs) labelled with QDs. Hybridization efficiency between NPs conjugated anti-senses and target nucleic acid hybrids was optimized and evaluated by the electrochemical analysis. The highest response of ODN-SH-NPs was determined forthe hybridization temperature of 25 °C. Further, we tested detection of the complexes via peak coming from the presence of ODNs and/or from the presence of metal ions in QDs. Under the optimized conditions, LODs (3 S/N) based on the dete

Název v anglickém jazyce

Using of paramagnetic microparticles and quantum dots for isolation and electrochemical detection of influenza viruses' specific nucleic acids

Popis výsledku anglicky

The aim of this study was to suggest isolation protocol based on paramagnetic microparticles and multicolour electrochemical detection of three various influenza oligonucleotides (H1N1 influenza subtype, H3N2 influenza subtype and H5N1 influenza subtype)modified with nanoparticles (NPs) called quantum dots (QDs), which are made from zinc, cadmium or lead. Particularly, we described hybridization assay based on paramagnetic microparticles (MPs) and coupled with detection of three different influenza derived oligonucleotides (ODNs) labelled with QDs. Hybridization efficiency between NPs conjugated anti-senses and target nucleic acid hybrids was optimized and evaluated by the electrochemical analysis. The highest response of ODN-SH-NPs was determined forthe hybridization temperature of 25 °C. Further, we tested detection of the complexes via peak coming from the presence of ODNs and/or from the presence of metal ions in QDs. Under the optimized conditions, LODs (3 S/N) based on the dete

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

14

Strana od-do

689-702

Kód UT WoS článku

316562800054

EID výsledku v databázi Scopus

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