Analysis of cadmium-phytochelatins 2 complexes using flow injection analysis coupled with electrochemical detection mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00200141" target="_blank" >RIV/62156489:43210/13:00200141 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/13:43872365 RIV/00216305:26620/13:PU103769

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of cadmium-phytochelatins 2 complexes using flow injection analysis coupled with electrochemical detection mass spectrometry

Popis výsledku v původním jazyce

In this study, in vitro synthesized Cd-phytochelatin (PC2) complexes were characterized using flow injection analysis/high performance liquid chromatography with CoulArray or Coulochem electrochemical detector of PC2 and Cd-PC2 complexes. Method of the formation of complex by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) was confirmed. Higher pH had lowered effect for Cd(II) detection but PC2 detection suffers much in task of sensitivity and for furtheranalysis we thus used the Britton-Robinson buffer at pH 2 as electrolyte. Higher temperature than 40 °C positively influenced the interaction between Cd and thiol groups of PC2 by Coulochem III. The time did not affect the forming of complex. The concentration had bigger effect on the formation of complex than time of incubation. The biggest change was, with regards to concentration, observed under applied concentration 400 ug/ml and where the slope was the most decreasing which means th

Název v anglickém jazyce

Analysis of cadmium-phytochelatins 2 complexes using flow injection analysis coupled with electrochemical detection mass spectrometry

Popis výsledku anglicky

In this study, in vitro synthesized Cd-phytochelatin (PC2) complexes were characterized using flow injection analysis/high performance liquid chromatography with CoulArray or Coulochem electrochemical detector of PC2 and Cd-PC2 complexes. Method of the formation of complex by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) was confirmed. Higher pH had lowered effect for Cd(II) detection but PC2 detection suffers much in task of sensitivity and for furtheranalysis we thus used the Britton-Robinson buffer at pH 2 as electrolyte. Higher temperature than 40 °C positively influenced the interaction between Cd and thiol groups of PC2 by Coulochem III. The time did not affect the forming of complex. The concentration had bigger effect on the formation of complex than time of incubation. The biggest change was, with regards to concentration, observed under applied concentration 400 ug/ml and where the slope was the most decreasing which means th

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

13

Strana od-do

4409-4421

Kód UT WoS článku

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EID výsledku v databázi Scopus

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