Characterisation of actinomycetes community from the heavy metals-polluted soil
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00205537" target="_blank" >RIV/62156489:43210/13:00205537 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.11118/actaun201361051471" target="_blank" >http://dx.doi.org/10.11118/actaun201361051471</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.11118/actaun201361051471" target="_blank" >10.11118/actaun201361051471</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterisation of actinomycetes community from the heavy metals-polluted soil
Popis výsledku v původním jazyce
The isolation of actinomycetes was performed from soil samples influenced by car-traffic. The acute toxicity of soil leaches was tested by the help of Microtox bioassay testing system which uses freeze dried luminescent bacteria Photobacterium phosphoreum as the test organisms. The content of heavy metals in biomass of soil microorganisms and in whole soil samples was determinate. 115 strains of actinomycetes were isolated and their total numbers in soil samples were estimated. The acute toxicity of soil influenced the total numbers of actinomycetes. By the help of DNA-DNA reassociation procedure the generic diversity of bacteria was estimated. The identification and differentiation of streptomycetes from the total isolated actinomycetes was made usingspecific morphological criteria and the gas chromatography-fatty acid methyl ester (GC-FAME) analysis. FAME method is adequate only for differentiation of members of genus Streptomyces from other actinomycetes because of their characteri
Název v anglickém jazyce
Characterisation of actinomycetes community from the heavy metals-polluted soil
Popis výsledku anglicky
The isolation of actinomycetes was performed from soil samples influenced by car-traffic. The acute toxicity of soil leaches was tested by the help of Microtox bioassay testing system which uses freeze dried luminescent bacteria Photobacterium phosphoreum as the test organisms. The content of heavy metals in biomass of soil microorganisms and in whole soil samples was determinate. 115 strains of actinomycetes were isolated and their total numbers in soil samples were estimated. The acute toxicity of soil influenced the total numbers of actinomycetes. By the help of DNA-DNA reassociation procedure the generic diversity of bacteria was estimated. The identification and differentiation of streptomycetes from the total isolated actinomycetes was made usingspecific morphological criteria and the gas chromatography-fatty acid methyl ester (GC-FAME) analysis. FAME method is adequate only for differentiation of members of genus Streptomyces from other actinomycetes because of their characteri
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
DO - Ochrana krajinných území
OECD FORD obor
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Návaznosti výsledku
Projekt
—
Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
ISSN
1211-8516
e-ISSN
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Svazek periodika
61
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
8
Strana od-do
1471-1478
Kód UT WoS článku
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EID výsledku v databázi Scopus
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