Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A00210092" target="_blank" >RIV/62156489:43210/13:00210092 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs

Popis výsledku v původním jazyce

The aim of this study was to evaluate the effect of age on sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs. We evaluated 90 semen samples. The dogs were divided into groups according to the age (A: 1.5--2 years, B: 2--5 years, C: 5--6.5 years). Semen samples were collected by manual manipulation into the glass beaker. Immediately after collection of macroscopic examination was made for all samples, which included find out volume of ejaculate, sperm activity,concentration and sperm cell viability. Volume was measured using the graduated cylinder. Concentration was evaluated by haematocytometry method using Bürker chamber and activity by subjective method according to the percentage of motile sperm in the native ejaculate. We evaluated the percentage of sperm with progressive direct movement after the head. Viability eosin-nigrosin stain method was performed for evaluation. In this case, we evaluated the total number of alive and dead sperms

Název v anglickém jazyce

Effect of age on the sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs

Popis výsledku anglicky

The aim of this study was to evaluate the effect of age on sperm activity, sperm cell viability and total number of spermatozoa in the ejaculate of dogs. We evaluated 90 semen samples. The dogs were divided into groups according to the age (A: 1.5--2 years, B: 2--5 years, C: 5--6.5 years). Semen samples were collected by manual manipulation into the glass beaker. Immediately after collection of macroscopic examination was made for all samples, which included find out volume of ejaculate, sperm activity,concentration and sperm cell viability. Volume was measured using the graduated cylinder. Concentration was evaluated by haematocytometry method using Bürker chamber and activity by subjective method according to the percentage of motile sperm in the native ejaculate. We evaluated the percentage of sperm with progressive direct movement after the head. Viability eosin-nigrosin stain method was performed for evaluation. In this case, we evaluated the total number of alive and dead sperms

Druh

D - Stať ve sborníku

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2013 - Proceedings of International PhD Students Conference

ISBN

978-80-7375-908-7

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

764-768

Název nakladatele

Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic

Místo vydání

Faculty of Agronomy

Místo konání akce

Mendelova univerzita v Brně

Datum konání akce

20. 11. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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